| MDH和G6PD同工酶在家鸡个体发育中的表现特征 |
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| 引用本文： | 马建岗,邱怀.MDH和G6PD同工酶在家鸡个体发育中的表现特征[J].西北农业学报,1995,4(1):61-65. |
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| 作者姓名： | 马建岗 邱怀 |
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| 摘 要： | 采用不连续缓冲系统的聚丙烯酰胺凝胶电泳法(PAGE法)结合组织特异性染色技术,对家鸡不同发育阶段和不同组织器官的苹果酸脱氢酶(MDH)和葡萄糖-6-磷酸脱氢酶(G6PD)同工酶进行了测定.结果表明,MDH同工酶在家鸡体内既有阶段发育特征,又有组织器官特异性表现,且s-MDH和m-MDH的分布和表达的量上有较大差别,表现在s-MDH在谱带多少、组织分布普遍程度和酶活性高低上都强于m-MDH.认为MDH同工酶在家鸡体内主要参与该组织器官的脂肪代谢.G6PD同工酶在家鸡未成年之前各发育阶段和各组织器官只呈现酶谱痕迹,成年之后仅在肝、心、肾、睾丸、胸肌中出现1～2条谱带,显示出分布的发育阶段和组织器官局限性.
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| 关 键 词： | MDH G6PD 家鸡 鸡 同功酶 个体发育 |
| 收稿时间： | 1994/4/21 0:00:00 |
EXPRESSIONAL CHARACTERISTICS OF MDH AND G6PD ISOZYMES IN THE ONTOGENY OF CHICKEN |
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| Ma Jiangang and Qiu Huai.EXPRESSIONAL CHARACTERISTICS OF MDH AND G6PD ISOZYMES IN THE ONTOGENY OF CHICKEN[J].Acta Agriculturae Boreali-occidentalis Sinica,1995,4(1):61-65. |
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| Authors: | Ma Jiangang and Qiu Huai |
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| Abstract: | The MDH and G6PD isozymes in chicken''s various developmental stages and different tissues and organs were investigated by using dissontinous PAGE method with tissue specific staining techniques.The results showed that MDH isozymes exhibited both stage developmental characteristics and tissue specific expression.Moreover,the distribution and amount of s-MDH and m-MDH had higher difference,e.g.the band number,the degree of distribution and the enzyme activity of s-MDH were stronger than that of m-MDH.It has been considered that MDH isozymes mainly participates the fat metabolism in which the isozyme presented based on the difference of metabolic characteristis of s-MDH and m-MDH within the body of animal.G6PD isozymes only presented the zymogram trace in various developmental stages with different tissues and organs in young chicken and 1 to 2 bands in liver,heart,kidney,tesitis and breast in adult chicken,appeared a distribution limitation in developmental staves with tissues and oreans. |
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| Keywords: | PAGE MDH G6PD Isozyme Chicken |
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