| 通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白（英文） |
| |
| 引用本文： | 姚清国,李晓芹,张文娜,周二鹏,王娟,王景翔.通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白（英文）[J].农业科学与技术,2010(11):77-81. |
| |
| 作者姓名： | 姚清国 李晓芹 张文娜 周二鹏 王娟 王景翔 |
| |
| 作者单位： | 石家庄学院化工学院,河北石家庄050035 |
| |
| 基金项目： | 2010年石家庄市科学技术研究与发展课题（10120803）;2007年石家庄学院科研基金立项课题（2007012）;2008年度石家庄学院教学改革研究项目（2008006）. |
| |
| 摘 要： | 目的]寻找CR4的胞外结合蛋白。方法]采用OsCR4的胞外域作为诱饵蛋白,酵母双杂交筛选14d的小苗cDNA文库。结果]得到了许多蛋白,包括1个过氧化物B（D26484）、甲硫氨酸硫氧还原酶（ABF96078）和1个未知功能的蛋白。结论]为研究CR4的信号转导机制提供了理论依据。
|
| 关 键 词： | 水稻 类受体激酶 胞外结合蛋白 酵母双杂交 |
Screening of Extracellular Binding Proteins of Rice Receptor-like Kinase CR4 by the Yeast Two-hybrid |
| |
| YAO Qing-guo,LI Xiao-qin,ZHANG Wen-na,ZHOU Er-peng,WANG Juan,WANG Jing-xiang.Screening of Extracellular Binding Proteins of Rice Receptor-like Kinase CR4 by the Yeast Two-hybrid[J].Agricultural Science ＆ Technology,2010(11):77-81. |
| |
| Authors: | YAO Qing-guo LI Xiao-qin ZHANG Wen-na ZHOU Er-peng WANG Juan WANG Jing-xiang |
| |
| Institution: | College of Chemical Engineering in Shijiazhuang University,Shijiazhuang 050035 |
| |
| Abstract: | Objective] The research aimed to find the extracellular binding proteins of CR4.Method] The extracellular domain of OsCR4 was as the bait protein,and the yeast two-hybrid was used to screen cDNA library of seedling which was cultivated 14 d.Result] A lot of proteins which included a peroxide B（D26484）,a methionine thioredoxin reductase（ABF96078）and an unknown function protein were gained.Conclusion] It provided the theory basis for studying the signal transduction mechanism of CR4. |
| |
| Keywords: | Rice Receptor-like kinase Extracellular binding protein Yeast two-hybrid |
| 本文献已被 维普 万方数据 等数据库收录！ |
