| 热激后冰浴时间与复苏时间对化学转化法转化效率的影响 |
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| 引用本文： | 郭姣洁,薛永常,薛张伟,徐书景,鞠建松.热激后冰浴时间与复苏时间对化学转化法转化效率的影响[J].农业科学与技术,2010(9):198-200. |
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| 作者姓名： | 郭姣洁 薛永常 薛张伟 徐书景 鞠建松 |
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| 作者单位： | [1]大连工业大学生物工程学院,辽宁大连116034 [2]河北师范大学生命科学学院,河北石家庄050016 |
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| 基金项目： | 河北省留学回国人员项目（2010年）;河北师范大学博士启动基金项目（L2009813）. |
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| 摘 要： | 目的]研究化学转化过程中热激后冰浴时间和复苏时间对转化效率的影响,确定简便快速的转化方法。方法]采用2种缓冲溶液制备感受态,以热激后冰浴时间或复苏时间为变量,研究这2个因素与转化效率的关系。结果]冰浴2min,复苏30或40min达到最佳转化效率;冰浴和复苏时间为0时仍能获得相当数量的转化子。结论]热激后冰浴和复苏时间对转化效率有一定影响,但不是决定性因素。因此,在一般性转化试验中可以省略这2个步骤以节省时间。
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| 关 键 词： | 感受态细胞 转化效率 冰浴时间 复苏时间 |
Effects of the Ice Bath Time after Heat Shock and the Incubation Time on the Efficiency of Chemical Transformation Method |
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| GUO Jiao-jie,XUE Yong-chang,XUE Zhang-wei,XU Shu-jing,JU Jian-song.Effects of the Ice Bath Time after Heat Shock and the Incubation Time on the Efficiency of Chemical Transformation Method[J].Agricultural Science ＆ Technology,2010(9):198-200. |
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| Authors: | GUO Jiao-jie XUE Yong-chang XUE Zhang-wei XU Shu-jing JU Jian-song |
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| Institution: | 1.College of Bio-engineering,Dalian Polytechnic University,Dalian 116034;2.College of Life Science,Hebei Normal University,Shijiazhuang 050016 |
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| Abstract: | Objective] The aim of the research was to study the effect of the ice bath time after heat shock and the incubation time on the transformation efficacy,and to establish a simple and quick transformation method.Method]Competent cells were prepared with two buffer solutions;with the ice bath time after heat shock and the recovery time as the variables,the relationship between these two factors and transformation efficacy was studied.Result]The transformation efficacy was the best when the ice bath time was 2 min and the recovery time was 30 or 40 min;when the ice bath time and the recovery time was 0 min,a certain amount of transformants still could be obtained.Conclusion]The ice bath time after heat shock and the recovery time had certain impact on transformation efficacy,but they were not the decisive factors.Therefore,in the general transformation experiment,these two steps could be omitted. |
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| Keywords: | Competent cell Transformation efficiency Ice bath time Recovery time |
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