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| 实时荧光定量PCR检测PrP基因在金黄地鼠脑组织的动态表达 | |
| 引用本文： | 宁章勇,赵德明,杨建民,周向梅,于博,孔小明,尹晓敏.实时荧光定量PCR检测PrP基因在金黄地鼠脑组织的动态表达[J].中国兽医杂志,2007,43(9):3-6. |
| 作者姓名： | 宁章勇 赵德明 杨建民 周向梅 于博 孔小明 尹晓敏 |
| 作者单位： | 1. 中国农业大学国家动物传染性海绵状脑病实验室,北京,海淀,100094;华南农业大学兽医学院,广东,广州,510642 2. 中国农业大学国家动物传染性海绵状脑病实验室,北京,海淀,100094 3. 华南农业大学兽医学院,广东,广州,510642 |
| 摘 要： | 金黄地鼠是研究动物传染性海绵状脑病的理想模型动物之一,其脑组织内朊蛋白基因动态表达数据的测定对探讨该类疾病的发生、发展和分子致病机理具有重要意义。我们利用实时荧光定量RT-PCR技术,对不同年龄金黄地鼠大脑、小脑、丘脑和脑干PrP基因的表达进行了定量。结果发现,脑的四个检测部位都呈现高的表达量,但是同一年龄段不同组织每纳克总RNA中朊蛋白基因的表达量和每毫克组织中朊蛋白基因的表达量有显著的差别,不同组织在不同年龄出现表达高峰。本研究的结果对于探讨朊蛋白的基本功能和脑组织在传染性海绵状脑病病理发生中的作用,提供了基础数据。 |
| 关 键 词： | 金黄地鼠 脑 朊蛋白 基因表达 |
| 文章编号： | 0529-6005（2007）09-0003-03 |
| 修稿时间： | 2005-12-07 |
Detection of prion gene in the brain of golden hamster detected by real-time RT-PCR |
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| NING Zhang-yong,ZHAO De-ming,YANG Jian-min,ZHOU Xiang-mei,YU Bo,KONG Xiao-ming,YIN Xiao-min.Detection of prion gene in the brain of golden hamster detected by real-time RT-PCR[J].Chinese Journal of Veterinary Medicine,2007,43(9):3-6. | |
| Authors: | NING Zhang-yong ZHAO De-ming YANG Jian-min ZHOU Xiang-mei YU Bo KONG Xiao-ming YIN Xiao-min |
| Institution: | 1. National Animal Transmissible Spongiform Encephalopathies Laboratory, China Agricultural University, Beijing 100094,China;2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642,China |
| Abstract: | Golden hamster is one of the favored experimental models for transmissible spongiform encephalopathies (TSEs). The data of prion gene dynamic expression in brain is crucial to discussion of the molecular mechanisms, occurrence and development of TSE. We used the real-time RT-PCR technique to quantify the PrP gene expression of cerebrum, cerebellum, thalamencephalon and brain stem from the golden hamster at different ages. The result showed that high mRNA levels were present in all four regions of the brain examined. But the copies per ng of total RNA and per mg of different tissues at the same age showed marked difference and the prion gene expression reached peak at different times for the different tissues. Our result provided the basic data for understanding the function of the PrPC and the role of different brain regions in TSEs. |
| Keywords: | Golden Hamster Brain Prion protein Gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |