| 白喉型鸡痘病毒Gibbs株免疫特性研究 |
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| 引用本文： | 朱庆虎,黄骏明,王翠兰.白喉型鸡痘病毒Gibbs株免疫特性研究[J].中国预防兽医学报,2001,23(1):39-42. |
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| 作者姓名： | 朱庆虎 黄骏明 王翠兰 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所, |
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| 摘 要： | 白喉型鸡痘病毒Gibbs株能在鸡胚绒毛尿囊膜上良好增殖，接种后１２０小时，绒毛尿囊膜明显水肿，且淡黄色痘斑，其毒价达到１０^5.25-10^5.83EID50/0.2ml。用１０倍稀释的病毒悬液肌肉注射１３日龄雏鸡，无任何不良反应。用１００倍稀释病毒液皮下刺种免疫１４－４０日龄鸡，接种后３－６天，接种局部出现痘痂，接种后１４天，用１０倍稀释的Ｇibbs株病毒液二次皮下刺种或用鸡痘野毒株经腿部毛作擦，免疫未见任何局部反应或其它异常，保护率达１００％。，免疫后１６５天，保护率仍达８０％，试验结果证实该毒株具有良好的免疫原性和安全性。
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| 关 键 词： | 免疫特性 白喉型鸡痘 GiBBs株 |
| 文章编号： | 1008-0589(2001)01-0039-04 |
| 修稿时间： | 2000年10月8日 |
Immunological
Characterization of Diphterin Type Fowl Pox Virus Gibbs Strain |
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| ZHU Qinghu,HUANG Junming,WANG Cuilan.Immunological
Characterization of Diphterin Type Fowl Pox Virus Gibbs Strain[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(1):39-42. |
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| Authors: | ZHU Qinghu HUANG Junming WANG Cuilan |
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| Abstract: | Gibbs strain,which is diphterin type fowl pox virus,may replicates well in 10-day-old embryonated chicken eggs via the dropped chorio allantoic membrane (CAM)route.120 hours post inoculation(pi),the swelling and pox reactions was observed in CAM.Moreover,the titer of virus suspersion is 105.25～105.83EID50 per 0.2ml.The minimal immune dose by the conventional wing-web route was 10-5 0.1ml.13-day-old chickens,inoculated by intramuscular injection with 1∶10 virus dilution,no abnormal signs was observed.Vaccinated chickens with 1∶100 dilution,developing lesion at site of inoculation.At 14 day post inoculation,these immuned chickens were challenged with a virulent fowl pox strain HL or Gibbs strain,which protective rate was 100 percent,provided excellent protection.The duration of immunity lasted 5.5 months. |
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| Keywords: | Immunological characterization Diphterin type pox Gibbs strain |
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