| 木薯细菌性枯萎病菌转座子库的构建及其质量评价 |
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| 引用本文： | 陈江莎,李超萍,祝天成,时涛,黄贵修.木薯细菌性枯萎病菌转座子库的构建及其质量评价[J].热带作物学报,2013,34(3):1144-1148. |
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| 作者姓名： | 陈江莎 李超萍 祝天成 时涛 黄贵修 |
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| 作者单位： | 1. 广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室 广西南宁530023
2. 广西农业科学院微生物研究所,广西南宁,530007 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费（No. ITBB110304）；海南省研究生创新科研课题（No. Hys2012-17）。 |
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| 摘 要： | 猫豆是广西种植的一种特色药用植物,近年来发生一些重要病害,为明确这些病害种类,对广西猫豆主产区进行主要病害种类调查和病原鉴定.每块地采取5点取样法进行取样调查,并通过形态特征和rDNAITS序列分析结果对病原菌进行鉴定.研究结果发现,猫豆上发生的主要病害有4种,分别为猫豆炭疽病(Colletotrichum gloeosporioides)、猫豆茎溃疡病(Phomopsis sp.)、猫豆斑枯病(Alternaria sp.)和猫豆褐斑病(Pseudocercospora sp.),这些病害的发病率达30％ ～80％.
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| 关 键 词： | 药用植物 猫豆 褐斑病 炭疽病 茎溃疡病 斑枯病 |
Purification of Chilli ringspot Virus and Preparation of Its Antiserum |
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| CHEN Jiangsh,LI Chaoping,ZHU Tiancheng,SHI Tao and HUANG Guixiu.Purification of Chilli ringspot Virus and Preparation of Its Antiserum[J].Chinese Journal of Tropical Crops,2013,34(3):1144-1148. |
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| Authors: | CHEN Jiangsh LI Chaoping ZHU Tiancheng SHI Tao and HUANG Guixiu |
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| Institution: | 1 College of Environment and Plant Protection, Hainan University
2 Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R. China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agri;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R. China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 College of Environment and Plant Protection, Hainan University
2 Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R. China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agri;1 College of Environment and Plant Protection, Hainan University
3 College of Agriculture, Hainan University;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R. China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, P.R. China, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | Velvet bean(Mucuna pruriens)is a kind of special medicinal plants grown in Guangxi. In recent years, some diseases were appeared on this plant and caused quality and yield loss. The diseases were investigated in 2012 in major planting areas of Guangxi. The pathogens were identified based on morphological characteristics and ITS DNA sequences analysis. Four diseases, brown spot, anthracnose, stem canker and spot blight were found, which were caused by Colletotrichum gloeosporioides, Phomopsis sp., Alternaria sp. and Pseudocercospora sp. respectively. The diseases incidences was ranged from 30% to 80%. |
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| Keywords: | Chilli ringspot virus Virus purification Polyclonal antibody ID-ELISA |
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