| 转BcWRKY Ⅰ基因玉米杂交组合苗期耐盐性分析 |
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| 引用本文： | 曹士亮,史桂荣,王成波,贺强,蔡泉,卢翠华,林忠平.转BcWRKY Ⅰ基因玉米杂交组合苗期耐盐性分析[J].黑龙江农业科学,2012(4):23-27. |
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| 作者姓名： | 曹士亮 史桂荣 王成波 贺强 蔡泉 卢翠华 林忠平 |
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| 作者单位： | 黑龙江省农业科学院玉米研究所;东北农业大学农学院;北京大学生命科学学院 |
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| 基金项目： | 农业部转基因重大专项资助项目(2009ZX08003-015B) |
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| 摘 要： | 为了明确外源基因提高玉米耐盐性的效果,以6份转BcWRKYⅠ基因的玉米杂交组合及其对应的6份非转基因对照组合为试材,对其发芽率、耐盐级别、株高、干重、脯氨酸含量等7个指标进行分析。结果表明:耐盐级别、发芽率、干重、叶绿素含量和相对电导率的变化均达到显著水平,株高的变化达到极显著水平,脯氨酸含量转基因与非转基因组合间差异不显著。
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| 关 键 词： | 转基因玉米 苗期 耐盐性 |
Analysis on Salt Tolerance of Transgenic Maize(Zea mays L.) Crosses with BcWRKY Ⅰ Gene in Seedling Stage |
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| CAO Shi-liang,SHI Gui-rong,WANG Cheng-bo,HE Qiang,CAI Quan,LU Cui-hua,LIN Zhong-ping.Analysis on Salt Tolerance of Transgenic Maize(Zea mays L.) Crosses with BcWRKY Ⅰ Gene in Seedling Stage[J].Heilongjiang Agricultural Science,2012(4):23-27. |
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| Authors: | CAO Shi-liang SHI Gui-rong WANG Cheng-bo HE Qiang CAI Quan LU Cui-hua LIN Zhong-ping |
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| Institution: | 1.Maize Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Hei-longjiang 150086;2.Agronomy College of Northeast Agricultural University,Harbin,Hei-longjiang 150030;3.Life Science College of Beijing University,Beijing 100083) |
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| Abstract: | In order to analyze the effect of salt tolerance improvement by exogenous gene transformation,six transgenic maize crosses with BcWRKY Ⅰ gene and six respective non-transgenic control of them were used to identify salt toleranc in seedling stage.Seven indicators such as the germination rate,salt resistance level,planth eight,dry weight,proline content and so on were analyzed to evaluate the effect of the transformation of Bc-WRKY Ⅰ gene on improving the salt resistance.The results indicated that salt resistance level,germination rate,dry weight,chlorophyll content,relative electric conductivity appeared significant differences,plant height appeared extremely significant differences,however,the difference of proline content was not significant. |
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| Keywords: | transgenic maize seedling stage salt resistance |
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