| 昆虫细胞工程研究进展 |
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| 引用本文： | 宋德伟,马艳,冯颖,陈晓鸣.昆虫细胞工程研究进展[J].林业科学研究,2004,17(1):116-124. |
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| 作者姓名： | 宋德伟 马艳 冯颖 陈晓鸣 |
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| 作者单位： | 中国林业科学研究院资源昆虫研究所,云南,昆明,650224 |
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| 基金项目： | 国家林业局948项目"昆虫细胞系及细胞系库建立技术引进"(2002 52) |
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| 摘 要： | 综述了从60年代首次建立昆虫细胞系以来国内外的昆虫细胞工程的研究进展。包括昆虫细胞培养基的研究,昆虫细胞系的建立,昆虫细胞冻存的研究,昆虫细胞培养过程中污染的检测和解决,以及昆虫细胞—杆状病毒表达系统(Baeulovinas Expression Vector System,BEVS)的研究现状和展望。
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| 关 键 词： | 昆虫细胞工程 培养基 细胞系 细胞冻存 杆状病毒表达系统 |
| 文章编号： | 1001-1498(2004)01-0116-09 |
| 收稿时间： | 2003/6/23 0:00:00 |
Advances in Research of Insect Cell Engineering |
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| SONG De-wei,MA Yan,FENG Ying and CHEN Xiao-ming.Advances in Research of Insect Cell Engineering[J].Forest Research,2004,17(1):116-124. |
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| Authors: | SONG De-wei MA Yan FENG Ying and CHEN Xiao-ming |
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| Institution: | Research Institute of Resource Insect, CAF, Kunming650224, Yunnan, China;Research Institute of Resource Insect, CAF, Kunming650224, Yunnan, China;Research Institute of Resource Insect, CAF, Kunming650224, Yunnan, China;Research Institute of Resource Insect, CAF, Kunming650224, Yunnan, China |
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| Abstract: | The advances in research of insect cell engineering at home and abroad since the establishment of the first insect cell line in 1960s were reviewed,which included the research of insect cell culture medium,establishment of insect cell lines,cryopresservation of insect cells,testing and solving of pollution during the course of the insect cell culture.The present research and future development of insect cell-baculovirus expression vector system were also discussed. |
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| Keywords: | insect cell culture insect cell lines pollution cryopreservation of cells baculovirus |
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