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Variation in seed protein digestion of different pea (Pisum sativum L.) genotypes by cecectomized broiler chickens: 1. Endogenous amino acid losses, true digestibility and in vitro hydrolysis of proteins |
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| Authors: | I Gabriel M Lessire H Juin J Burstin G Duc L Quillien JN Thibault M Leconte JM Hallouis P Ganier N Mzire B Sve |
| Institution: | aINRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France bINRA, UE1206 Elevage Alternatif et Santé des Monogastriques, Le Magneraud, BP52, F-17700 Saint-Pierre-d'Amilly, Surgères, France cINRA, Unité de Recherche Génétique et Ecophysiologie des Légumineuses, Domaine d'Époisses, F-21110 Bretenières, France dINRA, Unité de Recherche sur les Biopolymères, Interactions, Assemblages, rue de la Géraudière, F-44316 Nantes, France eINRA, UMR1079, Système d'Elevage Nutrition Animale et Humaine, F-35590 Saint Gilles, France |
| Abstract: | The aim of this study was to investigate the cause of variation in the digestibility of pea protein in poultry and to find a tool to select genotypes with high digestibility potential by using an in vitro hydrolysis assay. Eight pea genotypes were selected for their difference in seed protein content and composition. To reduce the variation due to tannins and particle size, seeds from these 8 genotypes were dehulled and micro-ground. They were incorporated as the only protein source in 8 different experimental isoproteinaceous diets with similar metabolisable energy content. The amino acid digestibility was studied in cecectomized chickens. A balance method was used to obtain apparent digestibility, and the isotope dilution technique was used to determine endogenous losses and true digestibility, after feeding a double labelled test meal containing chromic oxide and 15N-labelled peas. The 8 diets showed differences in apparent amino acid digestibility. The average apparent digestibility for all amino acids varied between 79.5 and 86.3%, with the highest values for arginine (85.2 to 90.8%) and glutamic acid (85.2 to 90.5%), and the lowest values for cystine (63.3 to 69.7%) and tryptophan (69.1 to 80.3%). This variability of apparent amino acid digestibility was due to variations in endogenous losses and true digestibility among the 8 pea genotypes. The average endogenous losses as determined for 9 amino acids ranged from 3.6 to 5.4% of ingested amino acids, with the highest value for threonine (8.0 to 11.0%). The average true digestibility varied between 84.4 and 90.2%, with the highest values for lysine (89.0 to 95.0%), and the lowest for isoleucine (81.0 to 88.7%) and valine (82.4 to 88.7%). In vitro hydrolysis of protein from micro-ground seeds was performed for the 8 pea genotypes using three proteases (pepsin, trypsin and chymotrypsin). The quantity of small peptides (< 3 kDa) that appeared after the combined hydrolysis with pepsin (3 h) followed by trypsin and chymotrypsin (15 min) was significantly correlated with the average true digestibility of the 8 genotypes (R = 0.74; P < 0.05). |
| Keywords: | Broiler chickens Cecectomy Pisum sativum Amino acids Digestibility Endogenous losses In vitro hydrolysis |
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