| 水稻抗稻瘟病基因Pi-ta和Pi-b多重PCR体系的构建与应用 |
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| 引用本文： | 姚姝,刘燕清,张亚东,朱镇,陈涛,赵庆勇,周丽慧,赵春芳,于新,王才林.水稻抗稻瘟病基因Pi-ta和Pi-b多重PCR体系的构建与应用[J].作物学报,2014,40(9):1565-1571. |
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| 作者姓名： | 姚姝 刘燕清 张亚东 朱镇 陈涛 赵庆勇 周丽慧 赵春芳 于新 王才林 |
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| 作者单位： | 江苏省农业科学院粮食作物研究所 / 江苏省优质水稻工程技术研究中心 / 国家水稻改良中心南京分中心, 江苏南京 210014 |
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| 基金项目： | 本研究由江苏省农业科技自主创新资金项目[CX(12)1003], 江苏省科技支撑计划项目(BE2013301)和国家现代农业产业技术体系建设专项(CARS-01-47)资助。 |
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| 摘 要： | 稻瘟病是我国水稻主产区的重要病害之一, 其主效抗性基因Pi-ta和Pi-b在我国很多稻区表现广谱持久的稻瘟病抗性, 被广泛应用于我国的水稻育种和生产。本研究选用稻瘟病抗性基因Pi-ta和Pi-b及其等位基因的功能标记, 在对22份分别已知抗病基因Pi-ta和Pi-b以及感病基因pi-ta与pi-b组成的水稻品种检测验证基础上, 建立了2套稻瘟病基因多重PCR体系: 体系I同时检测抗病基因Pi-ta与Pi-b, 体系II 同时检测感病基因pi-ta与pi-b, 并利用2套体系对336份高世代育种材料进行检测, 与单标记检测结果比较, 表现稳定可靠, 重复性好。本研究构建的抗稻瘟病基因分子标记多重PCR体系可用于水稻种质资源的快速评价和抗稻瘟病分子标记辅助育种。
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| 关 键 词： | 稻瘟病 分子标记 抗性基因 多重PCR体系 |
| 收稿时间： | 2014-02-07 |
Establishment and Application of Multiplex PCR System for Two Blast Resistance Genes Pi-ta and Pi-b in Rice |
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| YAO Shu,LIU Yan-Qing,ZHANG Ya-Dong,ZHU Zhen,CHEN Tao,ZHAO Qing-Yong,ZHOU Li-Hui,ZHAO Chun-Fang,YU Xin,WANG Cai-Lin.Establishment and Application of Multiplex PCR System for Two Blast Resistance Genes Pi-ta and Pi-b in Rice[J].Acta Agronomica Sinica,2014,40(9):1565-1571. |
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| Authors: | YAO Shu LIU Yan-Qing ZHANG Ya-Dong ZHU Zhen CHEN Tao ZHAO Qing-Yong ZHOU Li-Hui ZHAO Chun-Fang YU Xin WANG Cai-Lin |
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| Institution: | Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu High Quality Rice R&D Center / Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China |
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| Abstract: | Rice blast is one of the important diseases in major rice producing areas of our country. The main blast resistance genes Pi-ta and Pi-b showed good resistance spectrum to rice blast in many rice growing areas of China, and has been utilized in rice breeding and commercial production. In this study, two sets of multiple PCR system for the two rice blast genes were established by selecting the functional markers of two resistance genes of Pi-ta and Pi-b and two susceptible genes of pi-ta and pi-b, respectively, based on the verification of the genotype of the two rice blast genes known in 22 rice varieties. System I could detect the two resistance genes of Pi-ta and Pi-b, while system II could detect the two susceptible genes of pi-ta and pi-b. Genotypes of the two rice blast genes in 336 new japonica lines were detected by using the two sets of multiple PCR system. The results were highly consistent with those tested by the conventional single mark detection method, indicating that the two multiplex PCR systems were stable, reliable and time-saving. They may serve as a rapid and efficient method to identify and screen rice germplasm resources and be applied in marker assistant selection for pyramiding multiple genes (QTLs) of rice blast resistance in rice breeding. |
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| Keywords: | Rice blast Molecular marker Resistance gene Multiplex PCR system |
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