| 小豆荚色及粒重性状的RAPD分子标记初探 |
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| 引用本文： | 白璐,金文林,赵波,濮绍京.小豆荚色及粒重性状的RAPD分子标记初探[J].北京农学院学报,2005,20(1):18-22. |
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| 作者姓名： | 白璐 金文林 赵波 濮绍京 |
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| 作者单位： | 1. 北京市农业应用新技术重点实验室、北京农学院作物遗传育种研究所,北京,102206;新疆农业大学农学院,乌鲁木齐,830052
2. 北京市农业应用新技术重点实验室、北京农学院作物遗传育种研究所,北京,102206 |
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| 基金项目： | 北京市自然科学基金
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北京市重点实验室基金 |
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| 摘 要： | 将JN5×HB801杂交组合F2世代单株及其双亲按荚色及籽粒大小进行分组,荚色分为黑荚、褐荚和白荚3组;籽粒分为相对大粒型组和相对小粒型组,每组随机抽取12个单株DNA组成其BSA池,利用520条RAPD随机引物对BSA池及其亲本进行多态性检测。初步研究结果表明:S91在亲本及粒重基因池之间表现多态性;而引物S136在亲本及不同荚色基因池之间表现多态性。
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| 关 键 词： | 小豆 RAPD 荚色 粒重 BSA池 |
| 文章编号： | 1002-3186(2005)01-0018-05 |
| 修稿时间： | 2004年10月10日 |
Preliminarily Study on RAPD Markers of Pod Colour and Seed Weight Traits of Adzuki Bean (Vigna angularis) |
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| Bai Lu,JIN Wen-lin,ZHAO Bo,PU Shao-jing.Preliminarily Study on RAPD Markers of Pod Colour and Seed Weight Traits of Adzuki Bean (Vigna angularis)[J].Journal of Beijing Agricultural College,2005,20(1):18-22. |
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| Authors: | Bai Lu JIN Wen-lin ZHAO Bo PU Shao-jing |
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| Abstract: | In this experiement,F_(2) plants derived from cross between JN5 and HeBei801 were divided into groups by seed colour and seed weight,namely black pod ,brown and white pod in seed colour trait, and relatively big seeds and relatively small seeds in seed weight trait. DNAs of 12 random sampling plants from each group were utilized for Bulked segregant analysis(BSA).Genetic polymorphism within genepool and between parents had been extensively examined by 520 RAPD primers. The results preliminarily showed that one primer(S91) detected polymorphic band within genepool of seed weight and between parents, and the other primer(S136)detected polymorphic band within genepool of legume colour and between parents. |
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| Keywords: | adzuki bean(Vigna angularis) RAPD(Random amplified polymorphic DNA) legume colour bean weight bulked segregant analysis(BSA)pool |
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