Colloid in the anterior pituitary of helmet guinea fowl (<Emphasis Type="Italic">Numida meleagris galeata</Emphasis>): Morphometric analysis and pattern of occurrence in relation to apoptosis |
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| Authors: | Claudius Luziga Maulilio John Kipanyula Gabriel Mbassa Mamba Koichi |
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| Institution: | (1) Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3016, Morogoro, Tanzania;(2) Department of Veterinary Science, Faculty of Agriculture, Yamaguchi University, 753–8515 Yamaguchi, Japan |
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| Abstract: | Colloidal accumulations in the pars distalis of helmet guinea fowls at various ages from 1 to 450 days were examined by Periodic
acid-Schiff reaction, immunohistochemistry and electron microscopy. Round, ovoid and elongated colloids were observed. Colloids
(69.5 ± 2.997) with 0.169 ± 0.014 μm mean diameter were already present in a 1-day-old bird. Numerous colloids were encountered
in 450 days old birds (2931.333 ± 29.847) with 2.263 ± 0.078 μm mean diameter of round colloids. A significant difference
in the mean colloidal number and diameter between young and adult birds was observed. In young birds (aged 1–30 days) both
Periodic acid-Schiff reaction positive colloids and S-100 positive folliculostellate (FS) cells were found to appear first
on or near the posterolateral region. In adult birds, FS cells were found to completely surround the colloids. We examined
the biochemical components of colloids and the relationship with apoptosis by immunohistochemistry. Results showed that the
colloids are composed of clusterin protein. Apoptotic cells detected by single stranded DNA (ssDNA) were abundant and localized
preferentially near colloids. To define clearly the type of cells undergoing apoptosis in the anterior pituitary, we performed
electron microscopy. Numerous endocrine cells at different stages of apoptosis were found engulfed by FS cells that were in
close association with the colloidal accumulations. The occurrence of extremely large number of colloids in relation to apoptotic
profiles in anterior pituitary of helmet guinea fowl is discussed. |
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