| 长穗偃麦草EeSnRK2.6基因克隆及生物信息学分析 |
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| 引用本文： | 徐 蓓,郭丽香,赵昌平,高世庆,唐益苗.长穗偃麦草EeSnRK2.6基因克隆及生物信息学分析[J].麦类作物学报,2012,32(1):36-43. |
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| 作者姓名： | 徐 蓓 郭丽香 赵昌平 高世庆 唐益苗 |
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| 作者单位： | 1. 首都师范大学生命科学学院,北京,100048
2. 北京杂交小麦工程技术研究中心,北京,100097 |
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| 基金项目： | 国家抗逆转基因重大专项（2008ZX08002002）；北京市科技新星项目（2007B056、2008B035）；北京市自然基金项目（5102016）；北京农林科学院院青年基金项目。 |
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| 摘 要： | 蔗糖非发酵相关蛋白激酶家族2(SnRK2)基因家族受各种逆境胁迫诱导表达,在提高植物的抗逆性中发挥着至关重要的作用。为继续深入挖掘有效的抗逆相关基因资源并为转基因小麦提供重要候选基因,基于同源克隆方法从抗旱、耐盐性极强的长穗偃麦草中克隆了EeSnRK2.6基因的cDNA序列,该基因全长1 096bp,编码364个氨基酸,推测分子量为41.73kD,等电点为5.98,是亲水性氨基酸,共有18处磷酸化位点和1处跨膜域。氨基酸序列同源性分析发现,长穗偃麦草EeSnRK2.6基因与其他目前已报道的抗逆效果显著的SnRK2家族基因有较高同源性,同大豆SnRK2.6基因同源性达到95%,且具有典型的丝氨酸/苏氨酸类蛋白激酶保守域。系统进化树分析表明,EeSnRK2.6与SbSnRK2.6属于同一进化分枝上。因此,推测该基因属于SnRK2基因家族成员,可能在参与逆境胁迫反应、增强植物的抗逆性中起着重要作用。
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| 关 键 词： | 长穗偃麦草 SnRK 基因克隆 序列分析 系统进化树 |
Cloning and Bioinformatic Analysis of the SnRK2.6 Gene from Elytrigia elongate |
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| XU Bei,GUO Li xiang,ZHAO Chang ping,GAO Shi qing,TANG Yi miao.Cloning and Bioinformatic Analysis of the SnRK2.6 Gene from Elytrigia elongate[J].Journal of Triticeae Crops,2012,32(1):36-43. |
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| Authors: | XU Bei GUO Li xiang ZHAO Chang ping GAO Shi qing TANG Yi miao |
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| Institution: | 1.College of Life Science,Capital Normal University,Beijing 100048,China;2.Beijing Hybrid Wheat Engineering and Technology Research Center,Beijing 100097,China) |
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| Abstract: | Drought,salinity and low temperature are the major factors limiting crop′s productivity and quality.At present,there were a few of excellent genes used in research of transgenic crops with stress tolerance,which heavily restricted the research progress of the new materials and new germplasm of transgenic crops.Sucrose non-fermenting 1-related protein kinase 2(SnRK2) was induced to express by kinds of hyperosmotic stresses,played a key role in improving stress resistance of plants.Hence,in order to mine the effect of stress-related genes and supply important candidate genes for transgenic wheat,EeSnRK2.6 was cloned from Elytrigia elongate,which with good drought and salt-resistances,based on homologous cloning method in this study.The results showed that the cDNA of EeSnRK2.6 was 1096 bp in size and encoded 364 amino acids,encoding a hydrophilic amino acid with 18 phosphorylation sites and one membrane-spanning domain.The molecular weight and the iso-electric point was 41.73 kD and 5.98,respectively.The deduced amino acids of EeSnRK2.6 shared high homology to other genes of SnRK2,which displayed significant effect in stress tolerance,especially the deduced amino acid showed the maximum homology with SbSnRK2.6(95% identity),and it had typically conserved domain of serine/threonine kinase.The phylogenic tree showed that EeSnRK2.6 and SbSnRK2.6 belonged to the same group.Therefore,we deduced that the EeSnRK2.6 gene was a member of SnRK2 family and might participated in abiotic stress responses,which played important roles in enhancing the stress resistances of plant. |
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| Keywords: | Elytrigia elongate SnRK Gene cloning Sequence analysis Polygenetic tree |
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