Identification and evaluation of antioxidant activities of bamboo extracts |
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| Authors: | Mu?Jun Email author" target="_blank">Uehara?Tohru?Email author Li?Jianzhang Furuno?Takeshi |
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| Institution: | 1. Faculty of Science and Engineering,Shimane University,Matsue 690-8504,Japan
2. College of Material Sciences and Technology,Beijing Forestry University,Beijing 100083,P. R. China |
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| Abstract: | The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the inhibition activity
for peroxidation of linoleic acid, and the reduction power. The methanol-extracts of moso bamboo culms and madake bamboo leaves
presented stronger antioxidant activity compared with DPPH scavenging activity. Methanol-extract of moso bamboo culms was
further fractionated by different solvents and n-butanol soluble fraction exhibited the most significant activity in the DPPH scavenging assay. The fractionation of n-butanol soluble extract was isolated by silica gel column with gradient mixture solvent of chloroform and methanol. The isolated
fractions were directed by the antioxidant activity measured by scavenging the stable DPPH free radical. It was observed that
most of the eluted fractions showed the antioxidative activity. Fractions acquired from elution with the mixture solvent of
chloroform and methanol (10:1–5:1) showed stronger antioxidant activity than the other fractions. |
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| Keywords: | bamboo extract moso bamboo antioxidant activity DPPH radical |
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