| 巢式PCR法鉴定猪早期胚胎性别的研究 |
| |
| 引用本文： | 彭礼繁,罗光彬,白文林.巢式PCR法鉴定猪早期胚胎性别的研究[J].畜禽业,2007(5):14-17. |
| |
| 作者姓名： | 彭礼繁 罗光彬 白文林 |
| |
| 作者单位： | 沈阳农业大学畜牧兽医学院动物遗传与繁育实验室,辽宁,沈阳,110161 |
| |
| 摘 要： | 根据猪SRY基因序列设计合成2对巢式PCR引物作为性别鉴定引物,根据猪β-珠蛋白基因序列设计了一对引物作为内标引物建立了猪胚胎性别鉴定的PCR反应体系。公猪可以扩增出187bp的SRY基因片段和255bp的β-珠蛋白基因片段,母猪只能扩增出255bp的β-珠蛋白基因片段。由于巢式PCR只需3～8个细胞就可以在紫外灯下看到扩增结果,而常规PCR则需要较多的细胞,所以胚胎性别鉴定时使用巢式PCR效果更好。
|
| 关 键 词： | 巢式PCR 猪 早期胚胎 性别鉴定 |
| 文章编号： | 1008-0414(2007)05(N)-0014-04 |
| 修稿时间： | 2007年3月6日 |
Study on Using Nested PCR for Sex Determination of pig Early Embryos |
| |
| PENG Li-fan,LUO Guang-bin,BAI Wen-lin.Study on Using Nested PCR for Sex Determination of pig Early Embryos[J].Livestock and Poultry Industry,2007(5):14-17. |
| |
| Authors: | PENG Li-fan LUO Guang-bin BAI Wen-lin |
| |
| Abstract: | We designed 2 pairs of SRY gene nested primers for sex determination and a pair of β-globin gene primer as an internal standard in this study and established the system of PCR.As a result,187 bp and 255 bp bands could be visible on the gel in ultraviolet transilluminator for male,but for female,only 255 bp band could be visible.It needed only 3 to 8 cells for obtaining amplification result by nested PCR,but more cells were needed by normal PCR.So we could obtain better results of sex determination of bovine preimplantation embryos by nested PCR. |
| |
| Keywords: | Nested PCR Pig Early embryo Sex determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
