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水杨酸对盐胁迫下商洛黄芩生理特性的影响
引用本文:李小玲,华智锐.水杨酸对盐胁迫下商洛黄芩生理特性的影响[J].河南农业科学,2016(3):116-119.
作者姓名:李小玲  华智锐
作者单位:商洛学院 生物医药与食品工程学院,陕西 商洛,726000
基金项目:陕西省科技厅科研项目(2009K01-11),陕西省商洛市科技局科研项目
摘    要:在5 g/L NaCl胁迫下,测定了不同浓度(0、0.5、1.0、1.5、2.0、2.5 mmol/L)水杨酸(SA)处理下黄芩种子的发芽势、发芽率、发芽指数,以及幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、叶片叶绿素和丙二醛(MDA)含量,研究不同浓度SA对盐胁迫下商洛黄芩种子萌发及幼苗生长特性的影响。结果表明,5 g/L NaCl胁迫下的黄芩种子萌发受到明显抑制,经0.5~2.5 mmol/L SA处理后,萌发指标均有升高。以1.0 mmol/L SA处理效果最佳,其种子的发芽率(28.7%)、发芽指数(17.4)、发芽势(23.0%)均比盐对照组(CK_2)高,且达到最大值;SOD、POD活性分别是CK_2的1.52倍和7.56倍;叶绿素含量达CK2的1.45倍。说明适宜浓度的SA能够有效地缓解盐害,提高黄芩的抗盐性,以1.0 mmol/L SA处理效果最佳。

关 键 词:商洛黄芩  水杨酸  盐胁迫  生理特性

Effects of Salicylic Acid on Physiological Characteristics of Shangluo Scutellaria baicalensis under Salt Stress
Abstract:In order to explore the effects of salicylic acid ( SA ) on seed germination and physiological characters of Shangluo Scutellaria baicalensis under salt stress, under 5 g/L NaCl stress condition, the effects of SA with different concentrations on the seed germination rate,and skullcap superoxidedismutase (SOD),peroxidase(POD) activity,chlorophyll content of leaves,malondialdehyde(MDA) content were investigated. The results showed that the seed germination of Shangluo Scutellaria baicalensis under 5 g/L NaCl stress was significantly inhibited,and the germination index increased after the treatment with 0. 5—2. 5 mmol/L SA,the effect of 1. 0 mmol/L SA treatment was best. For the treatment with 1. 0 mmol/L SA,the seed germination rate(28. 7%),germination index(17. 4) and germination potential(23. 0%) were higher than the salt control group(CK2),and achieved the maximum; the SOD,POD activity were 1. 52 and 7. 56 times the values of CK2;the chlorophyll content was 1. 45 times the value of CK2 . There-fore,moderate SA could mitigatethe injuries induced by the NaCl stress,and improve the salt resistance of Shangluo Scutellaria baicalensis,the effect of the treatment with 1. 0 mmol/L SA was the best.
Keywords:Shangluo Scutellaria baicalensis  salicylic acid  salt stress  physiological characteris
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