| 陕西省规模猪场副猪嗜血杆菌的分离鉴定及敏感药物筛选 |
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| 引用本文： | 张勇,赵兴绪.陕西省规模猪场副猪嗜血杆菌的分离鉴定及敏感药物筛选[J].河南农业科学,2016(4):126-129. |
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| 作者姓名： | 张勇 赵兴绪 |
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| 作者单位： | 甘肃农业大学 动物医学院,甘肃 兰州,730070 |
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| 摘 要： | 为了解陕西省部分规模猪场副猪嗜血杆菌株的血清型及耐药特性,以指导临床用药,防控副猪嗜血杆菌病,对陕西省3个规模化猪场共60头表现副猪嗜血杆菌症状的病猪进行病理剖检,采集肝脏、肺脏、淋巴结、关节液等进行细菌分离培养及生化试验,并对分离菌株进行耐药性试验。结果表明,分离到42株副猪嗜血杆菌,其中血清1型1株(2.38%),4型8株(19.05%),5型15株(35.71%),12型3株(7.14%),13型11株(26.19%),其他型1株(2.38%),未定型的有3株(7.14%)。药敏试验结果表明,分离菌株对阿奇霉素、复方新诺明、氟苯尼考、洛美沙星高度敏感,对左旋氧氟沙星、替米考星、庆大霉素、诺氟沙星、磺胺甲氧嘧啶中度敏感,对头孢噻呋钠、链霉素、红霉素、林可霉素、环丙沙星、强力霉素不敏感。
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| 关 键 词： | 副猪嗜血杆菌 分离 鉴定 药敏试验 陕西省 |
Isolation,Identification and Medicine Sensitivity Test of Haemophilus parasuis in Scale Pig Farm |
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| Abstract: | In order to investigate the serotype and drug resistance of Haemophilus parasuis in some large scale pig farms in Shaanxi province,as well as to provide basis for prevention and control measures,this test carried out a pathological examination of 60 sick pigs from three large scale farms. The bacteria isolated from liver, lung, lymph node and joint fluid were collected and cultivated for further drug resistance test. The results showed that 42 strains wereisolated, and among them one strain belonged to type 1(2. 38%),eight strains belonged to type 4(19. 05%),fifteen strains belonged to type 5(35. 71%), three strains belonged to type 12 ( 7. 14%), thirteen strains belonged to type 11 ( 26. 19%), one strain belonged to other type(2. 38%) and three strains belonged to uncertain type(7. 14%). Susceptibility test results showed that the isolated strains were highly sensitive to azithromycin, sulfamethoxazole, triimethoprim florfenicol and lomefloxacin, moderatelly sensitive to levofloxacin, tilmicosin, gentamicin, norfloxacin and sulfamonomethoxine, insensitive to ceftiofur sodium, streptomycin, erythromycin, lincomycin,ciprofloxacin and doxycycline. |
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| Keywords: | Haemophilus parasuis isolation identification medicine sensitivity test Shaanxi province |
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