| 毛竹花粉活力测定及其适宜保存条件研究 |
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| 引用本文： | 王青,彭祚登,郭起荣,冯云,孙立方.毛竹花粉活力测定及其适宜保存条件研究[J].竹子研究汇刊,2012(1):23-26,32. |
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| 作者姓名： | 王青 彭祚登 郭起荣 冯云 孙立方 |
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| 作者单位： | 北京林业大学;国际竹藤网络中心 |
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| 基金项目： | 国家十二五科技支撑“竹藤资源培育与高附加值加工利用技术研究”;国家自然科技资源条件平台“林木(含竹藤花卉)种质资源标准化整理、整合及共享试点” |
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| 摘 要： | 试验立足于毛竹花粉种质的保存,得出毛竹花粉萌发的适宜条件为25℃、15%蔗糖+0.001%硼酸培养1 h,萌发率可达92.5%。适合毛竹花粉较长时间保存的条件为:新采集的花药,室内阴干1 h,离心管密封保存于4℃冷藏室,可维持80 h的活力。零下低温保存毛竹花粉技术需要进一步探索。
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| 关 键 词： | 毛竹 花粉活力 种质保存 萌发率 |
Pollen Vitality Test and Conservation Methods of Moso Bamboo |
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| Wang Qing,Peng Zuodeng,Guo Qirong,Feng Yun,Sun Lifang.Pollen Vitality Test and Conservation Methods of Moso Bamboo[J].Journal of Bamboo Research,2012(1):23-26,32. |
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| Authors: | Wang Qing Peng Zuodeng Guo Qirong Feng Yun Sun Lifang |
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| Institution: | 1.Beijing Foresty University,Beijing 100083,China; 2.International Bamboo and Rattan Center,Beijing 100102,China) |
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| Abstract: | Aimed at the germplasm conservation of Moso pollen,this study explored the condition suited for its germination and conservation.The results indicated that the suitable conditons for its germination were 15% sugar + 0.001% boric acid at 25℃ for 1h.The germination rate could reach 92.5%.The suitable conditions of pollen conservation were that the new collecting anthers dried for 1h in room and then conserved in 4℃ freezer with sealed centrifuge tubes.That could maintain the vitality of pollen for 80h.The pollen conserved in subzero temperature and ultralow temperature(-196℃) lost their vitality.The technology of pollen preserved in subzero temperature needs further study. |
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| Keywords: | Moso Pollen vitality Germplasm conservation Germination rate |
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