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| 桑花叶型萎缩病病原的检测与序列分析 | |
| 引用本文： | 孔卫青,杨金宏.桑花叶型萎缩病病原的检测与序列分析[J].中国农学通报,2012,28(36):237-240. |
| 作者姓名： | 孔卫青 杨金宏 |
| 作者单位： | 安康学院/陕西省蚕桑重点实验室,陕西安康,725000 |
| 基金项目： | 陕西省科技厅农业攻关项目"抗花叶型萎缩病转基因桑树选育"，陕西省教育厅重点实验室项目"核酶在桑树类病毒病害防治中的应用" |
| 摘 要： | 旨在获得安康地区桑花叶萎缩病病原序列，为桑花叶萎缩病的防治提供更多的信息。用RT-PCR技术对安康地区染花叶型萎缩病病株的病原进行了分离，克隆测序并在Mfold网站预测其二级结构。该病的病原为桑花叶萎缩类病毒MMDVd-201110151（GenBank登录号：JQ809700），核苷酸长度357 bp，GC含量50.7%，219 bp后38个碱基与樱桃小圆形类病毒样核糖核酸具有约85%的一致性。Mfold网站预测二级结构有58.3%的碱基配对，两端为分枝状结构。成功分离到安康地区桑花叶型萎缩病病原。 |
| 关 键 词： | 产量 产量 |
| 收稿时间： | 2012/5/22 0:00:00 |
| 修稿时间： | 2012/7/22 0:00:00 |
The Detection and Sequence Analysis of Pathogen from Mulberry Mosaic Dwarf Disease |
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| Kong Weiqing , Yang Jinhong.The Detection and Sequence Analysis of Pathogen from Mulberry Mosaic Dwarf Disease[J].Chinese Agricultural Science Bulletin,2012,28(36):237-240. | |
| Authors: | Kong Weiqing Yang Jinhong |
| Institution: | (Ankang University/Shaanxi Provincial Key Laboratory of Sericulture,Ankang Shaanxi 725000) |
| Abstract: | The aims were to obtain the pathogenic sequence of mulberry mosaic dwarf disease in Ankang area, and provide more information on the control of the disease. The pathogen of mulberry mosaic dwarf disease in Ankang area was isolated by RT-PCR, and the secondary structure was predicted in Mfold web. The length of the pathogen, MMDVd-201110151 (GenBank accession number: JQ809700), was 357 bp, and the GC percent was 50.7% in it. The 38 bp behind 219 bp had 85% identity with cscRNA1.150. The secondary structure of MMDVd-201110151 was predicted in Mfold web, 58.3% pairs in the secondary structure, and the two ends in it were both branched. The pathogen of mulberry mosaic dwarf disease in Ankang area was isolated successfully. |
| Keywords: | secondary structure |
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