| 远红光补光对辣椒幼苗生长和非生物胁迫抗性的影响 |
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| 引用本文： | 董桑婕,姜小春,王羚羽,林锐,齐振宇,喻景权,周艳虹.远红光补光对辣椒幼苗生长和非生物胁迫抗性的影响[J].中国农业科学,2022,55(6):1189-1198. |
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| 作者姓名： | 董桑婕 姜小春 王羚羽 林锐 齐振宇 喻景权 周艳虹 |
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| 作者单位： | 1浙江大学农业与生物技术学院/农业部园艺植物生长发育重点实验室,杭州 3100582浙江大学农业试验站,杭州 310058 |
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| 基金项目： | 浙江省重点研发计划（2018C02010）；;国家现代农业产业技术体系（CARS-24-B-01）； |
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| 摘 要： | 【目的】研究补照适量远红光（FR）对辣椒幼苗生长发育和非生物胁迫抗性的调控作用,旨在为实际生产过程中利用精确的光环境调控手段培育壮苗提供理论依据。【方法】以辣椒‘博辣红帅’品种为研究材料,将苗龄7 d的辣椒幼苗置于LED光源对照光谱（NL;红R/蓝B=3/1,光量子通量密度PPFD为150 μmol·m-2·s-1）及在此基础上分别补充10 μmol·m -2·s-1远红光（6% FR）、20 μmol·m -2·s-1远红光（13% FR）和30 μmol·m -2·s-1远红光（20% FR）的处理组光谱环境条件下培养,并于苗龄21 d时进行低温和干旱处理。通过测定生物量、抗性相关基因表达、抗氧化酶活性、激素含量、叶绿素荧光参数以及叶片相对电导率等,探究补充6% FR对辣椒幼苗生长和非生物胁迫抗性的影响。【结果】与对照光谱相比,补充6% FR显著提高了辣椒幼苗株高、茎粗、干鲜重以及壮苗指数（P<0.05）。低温胁迫下,相比于对照光谱组,补充6% FR显著提高了辣椒叶片冷响应基因CBF1和抗氧化酶相关基因Cu/Zn-SOD、GR、APX、CAT、DHAR的表达水平,超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、脱氢抗坏血酸还原酶（DHAR）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）活性比对照分别增加25.2%、53.6%、55.8%、72.7%和33.4%,抗逆相关激素脱落酸（ABA）的含量提高69.5%。同时,低温胁迫下补充6% FR后辣椒叶片PSII最大光化学效率（Fv/Fm）较对照光谱组显著升高,而相对电导率（REL）显著降低,表明补充6% FR缓解了低温下PSII光抑制和叶片细胞的损伤,提高了辣椒幼苗的耐冷性。此外,干旱胁迫下,相比于对照光谱组,补充6% FR使辣椒幼苗的抗氧化酶SOD、GR、APX、CAT、DHAR活性分别增加13.7%、38.0%、37.2%、27.6%和23.7%,ABA含量和PSII实际光化学效率（ΦPSII）也显著升高,而REL则明显降低,表明补充6% FR减轻了干旱胁迫引起的PSII光抑制和膜脂过氧化,提高了辣椒幼苗的耐旱性。【结论】补充6% FR不仅可促使辣椒壮苗的形成,还可通过增加抗氧化酶活性和ABA含量提高辣椒幼苗对低温胁迫和干旱胁迫的抗性。
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| 关 键 词： | 辣椒 远红光 壮苗指数 低温胁迫 干旱胁迫 |
| 收稿时间： | 2021-06-23 |
Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings |
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| DONG SangJie,JIANG XiaoChun,WANG LingYu,LIN Rui,QI ZhenYu,YU JingQuan,ZHOU YanHong.Effects of Supplemental Far-Red Light on Growth and Abiotic Stress Tolerance of Pepper Seedlings[J].Scientia Agricultura Sinica,2022,55(6):1189-1198. |
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| Authors: | DONG SangJie JIANG XiaoChun WANG LingYu LIN Rui QI ZhenYu YU JingQuan ZHOU YanHong |
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| Institution: | 1Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University/State Agriculture Ministry Key Laboratory of Horticultural Plant Growth and Development, Hangzhou 3100582Agricultural Experimental Station, Zhejiang University, Hangzhou 310058 |
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| Abstract: | 【Objective】This study analyzed the effects of supplementary far-red light (FR) on the growth and abiotic stress tolerance of pepper seedlings, aiming to provide a theoretical basis on precise light environments for cultivating high quality vegetable seedlings.【Method】In this study, Bola Hongshuai pepper cultivar was used as the research material. The 7-day-old seedlings were cultivated under two LED light environments, including the control spectrum (NL; R/B = 3/1, 150 μmol?m -2?s-1 PPFD) and the NL with an extra 10 μmol?m -2?s-1far-red light (6% FR), 20 μmol?m -2?s-1far-red light (13% FR), and 30 μmol?m -2?s-1far-red light (20% FR). Chilling and drought were imposed when the seedlings were 21 days old. Biomass, resistance-related gene expression, antioxidant enzyme activity, hormone content, chlorophyll fluorescence parameters, and leaf relative electrolyte leakage (REL) were analyzed to explore the effects of supplemental FR on growth and abiotic stress tolerance of pepper seedlings. 【Result】 Compared with the control, the supplementation of 6% FR was beneficial to increase the height, stem thickness, dry weight, fresh weight and seedling indexes of pepper seedlings. Moreover, the supplementation of 6% FR significantly increased the expression of the cold response gene CBF1 and antioxidant enzyme-related genes, such as Cu/Zn-SOD, GR, APX, CAT and DHAR under chilling stress. The activity of SOD, APX, DHAR, CAT and GR as well as the ABA content of pepper seedlings under low temperature increased by 25.2%, 53.6%, 55.8%, 72.7%, 33.4% and 69.5%, respectively, following the treatment with supplemental FR. The PSII maximum photochemical efficiency (Fv/Fm) of pepper leaves after supplementation of 6% FR under low temperature stress significantly increased compared with the control, while REL decreased obviously, indicating that supplementation of 6% FR alleviated the low temperature-induced PSII photoinhibition and damage in leaves and enhanced the cold tolerance of pepper seedlings. Under the drought stress condition, compared with the control, the supplementation of 6% FR improved the activity of SOD, GR, APX, CAT and DHAR by 13.7%, 38.0%, 37.2%, 27.6% and 23.7%, respectively. The ABA content and PSII actual photochemical efficiency (ΦPSII) were both increased while REL was decreased significantly. The results showed that the supplementation of 6% FR reduced the degree of PSII photoinhibition and membrane lipid peroxidation caused by drought stress, and improved the drought tolerance of pepper seedlings.【Conclusion】The study found that supplementation of 6% FR could not only improve the quality of pepper seedlings, but also enhance the resistance of pepper seedlings to low temperature and drought stresses by improving antioxidant defense and ABA homeostasis. |
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| Keywords: | pepper (Capsicum annuum L ) far-red light seedling index low temperature stress drought stress |
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