| 广泛靶向代谢组学解析桃蚜危害对桃树次生代谢产物的影响 |
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| 引用本文： | 闫乐乐,卜璐璐,牛良,曾文芳,鲁振华,崔国朝,苗玉乐,潘磊,王志强.广泛靶向代谢组学解析桃蚜危害对桃树次生代谢产物的影响[J].中国农业科学,2022,55(6):1149-1158. |
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| 作者姓名： | 闫乐乐 卜璐璐 牛良 曾文芳 鲁振华 崔国朝 苗玉乐 潘磊 王志强 |
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| 作者单位： | 中国农业科学院郑州果树研究所,郑州 450009 |
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| 基金项目： | 国家自然科学基金（32071800,31701880）；;国家重点研发计划（2019YFD1000801）； |
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| 摘 要： | 目的]阐明桃蚜(Myzus persicae)危害条件下桃树抗蚜和感蚜品种的代谢响应机制,并确定桃树响应桃蚜危害过程中起关键作用的次生代谢产物.方法]采用抗蚜和感蚜桃树幼嫩新梢进行桃蚜危害处理,取接种3 d的桃树新梢进行次生代谢产物提取,并利用超高效液相色谱串联质谱(UPLC-MS/MS)对抗蚜品种(品系)'96-...
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| 关 键 词： | 桃树 桃蚜 抗性 代谢组学 次生代谢产物 超高效液相色谱串联质谱 |
| 收稿时间： | 2021-08-13 |
Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites |
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| YAN LeLe,BU LuLu,NIU Liang,ZENG WenFang,LU ZhenHua,CUI GuoChao,MIAO YuLe,PAN Lei,WANG ZhiQiang.Widely Targeted Metabolomics Analysis of the Effects of Myzus persicae Feeding on Prunus persica Secondary Metabolites[J].Scientia Agricultura Sinica,2022,55(6):1149-1158. |
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| Authors: | YAN LeLe BU LuLu NIU Liang ZENG WenFang LU ZhenHua CUI GuoChao MIAO YuLe PAN Lei WANG ZhiQiang |
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| Institution: | Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009 |
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| Abstract: | 【Objective】The objective of this study is to clarify the underlying biochemical mechanisms related to the resistance and susceptibility of peach towards Myzus persicae, and to identify the key secondary metabolites of peach responding to the M. persicae infection.【Method】New shoots of resistant (‘96-5-1’ (9651), ZaoYouTao (ZYT)) and susceptible (Zhong You 13 (CN13), Zhong Nong Jin Hui (ZNJH)) peach trees were inoculated with M. persicae for 3 days and used for secondary metabolite extraction and UPLC-MS/MS analysis. Differentially altered metabolites (DAMs) were screened with |log2 fold change|≥1, P-value≤0.01 as threshold. The VIP value of the OPLS-DA model was used to perform differences between resistant and susceptible peach.【Result】To illustrate the biochemical mechanisms of M. persicae resistance in peach, aphid-resistant/aphid-susceptible peach varieties infested with M. persicae for 3 days. A total of 528 metabolites were identified in the treated samples through widely targeted metabolomics analysis. Using principal component analysis (PCA), hierarchical cluster analysis (HCA) and Venn diagram analysis, it was found that 7 DAMs were identified from the susceptible variety CN13, and 2 of them were significantly decreased, 5 of them were significantly increased. 7 DAMs were identified from the susceptible variety ZNJH, and 3 of them were significantly decreased, 4 of them were significantly increased. 33 DAMs were identified from the resistant variety ‘96-5-1’, and 1 of them was significantly decreased, 32 of them were significantly increased. 55 DAMs were identified from the resistant variety ZYT, and 12 of them were significantly decreased, 43 of them were significantly increased. The majority of the DAMs were identified from the two resistant varieties, and the overall magnitude of change was greater in the resistant varieties than that in the susceptible varieties. Finally, 15 secondary metabolites (6 amino acids and their derivatives, 5 phenolic acids, 3 nucleotides and their derivatives, and 1 organic acid) were considered to be involved in M. persicae resistance of ‘Shou xing tao’.【Conclusion】The significantly up-regulated secondary metabolites obtained in M. persicae-resistant peach varieties were mainly involved in the response to M. persicae feeding. The regulation of these secondary metabolites (amino acids and their derivatives, phenolic acids, nucleotides and their derivatives, organic acid) is the important mechanism of defense reaction to M. persicae. |
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| Keywords: | peach green peach aphid (Myzus persicae) resistance metabolomics secondary metabolite UPLC-MS/MS |
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