Genetic variations of randomly amplified polymorphic DNA polymorphisms in Taoyuan and Duroc pigs |
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| Authors: | Yen Huang & Tai |
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| Institution: | Taiwan Livestock Research Institute, Shinhua, Tainan, Taiwan, R.O.C.;Department of Animal Science, National Chung Hsing University, Taichung, Taiwan, R.O.C.;Institute of Biotechnology, National Cheng Kung University, Tainan, Taiwan, R.O.C. |
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| Abstract: | The purposes of this study were to assess the genetic variability between Taoyuan (T) and Duroc (D) pigs using randomly amplified polymorphic DNA (RAPD) fingerprints and to evaluate the genetic relationship to a commercial synthetic line-Taiwan Black (TB) pig (75% D, 25% T). To assess the genetic variability between T and D, 71 random primers (Operon) were used for RAPD fingerprinting by polymerase chain reaction (PCR). The evaluation of the genetic relationship was based on band sharing frequency and band frequency. Thirty-five of the 71 primers (49%) could generate polymorphisms in RAPD fingerprints of T or D pigs. Twenty-two primers produced polymorphic bands from only T genomic DNA, and 14 primers could produce polymorphic bands from only D genomic DNA. These results indicated that there was some genetic difference between T and D pigs. The within-population genetic similarity (WGS) for T, D, and TB populations were 0.742, 0.747, and 0.745, respectively, the between-population genetic similarity (BGS) was 0.946 between T and TB; 0.953 between D and TB; and 0.934 between D and T. The parameters of genetic distance between T and TB; D and TB, T and D were 0.080, 0.064, and 0.096, respectively. When the values of genetic similarity and genetic distance between populations estimated as frequency of occurrence of bands showed lower genetic similarities between pig populations, but indicted similar relationship. TB was genetically more related to D than to T. It provided evidence of the usefulness of the RAPD technique to determine genetic relatedness among T, D, and TB. |
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