| 抗猪瘟兔化弱毒单克隆抗体杂交瘤细胞株的建立 |
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| 引用本文： | 黄宪高,余克伦.抗猪瘟兔化弱毒单克隆抗体杂交瘤细胞株的建立[J].广西农业生物科学,1994(2). |
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| 作者姓名： | 黄宪高 余克伦 |
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| 作者单位： | 广西农业大学牧医系 |
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| 摘 要： | 用猪瘟兔化弱毒（ＳＦＶ—Ｃ）牛睾丸细胞培养上清液，经ＰＥＧ—２００００沉淀和蔗糖密度梯度离心提纯制备ＳＦＶ—Ｃ抗原，腹腔免疫Ｂａｌｂ／ｃ小鼠，取抗体阳性的免疫小鼠脾细胞，在５０％（Ｗ／Ｖ）ＰＥＧ—４０００作用下，与ＳＰ２／０－Ａｇ１４小鼠骨髓瘤细胞进行融合，产生杂交瘤细胞。用间接ＥＬＩＳＡ法筛选阳性杂交瘤细胞株。通过有限稀释进行３次克隆，获得了２株分泌抗ＳＦＶ—Ｃ的单克隆抗体杂交瘤细胞株，并对其染色体形态和数目进行分析鉴定。
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| 关 键 词： | 猪瘟兔化弱毒中国株 酶联免疫吸附测定 单克隆抗体 杂交瘤细胞株 |
ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES AGAINST LAPINIZED CHINESE STRAIN OF SWINE FEVER VIRUS |
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| Huang Xiangao,Yu Kelun.ESTABLISHMENT OF HYBRIDOMA CELL LINES SECRETING MONOCLONAL ANTIBODIES AGAINST LAPINIZED CHINESE STRAIN OF SWINE FEVER VIRUS[J].Journal of Guangxi Agricultural and Biological Science,1994(2). |
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| Authors: | Huang Xiangao Yu Kelun |
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| Abstract: | Lapinized chinese strain of swine fever virus (SFV-C) from the supernatant of bovine testis cell culture was concentrated by precipitation with polyethylene glycol(M.W.20000) and purified by sucrose density gradient centrifugation. Balb/c mice were inoculated three times intraperitoneally with SFV-C purified. Hybrid cells were produced by fusion of spleen cells of Balb/c mouse with positive serum and mouse myeloma cells SP2/0-Ag14 using 50% (W/V) PEG (M.W.4000). Positive hybridoma cells were selected by screening their supernatants with an indirect enzyme-linked immunosorbent assay.Two hybridoma cell lines secreting monoclonal antibodies against SFV-C were estalished after 3 clonings by limiting dilution. The morphologieal features as well as the numbers of the hybridomas nucleic chromosomes were identified. |
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| Keywords: | Lapinized chinese strain of swine fever virus enzyme-linked immunosorbent assay monoclonal antibody hybridoma cell line |
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