| 鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响 |
| |
| 引用本文： | 黄怡,黄琴,李卫芬,余东游,周绪霞.鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响[J].畜牧兽医学报,2012,43(2):250-254. |
| |
| 作者姓名： | 黄怡 黄琴 李卫芬 余东游 周绪霞 |
| |
| 作者单位： | 1. 浙江大学动物科学学院动物分子营养学教育部重点实验室,杭州310058;广西大学动物科学技术学院,南宁530005
2. 浙江大学动物科学学院动物分子营养学教育部重点实验室,杭州,310058 |
| |
| 基金项目： | 浙江省重大科技专项，国家重点基础研究发展计划，博士学科点专项科研基金 |
| |
| 摘 要： | 本研究旨在探明鼠李糖乳杆菌(L rhamnosus)对Caco-2细胞抗氧化和非特异免疫功能的影响.Caco-2 细胞分别用PBS(A组)、E.coli K88(B组)和L.rhamnosus(C组)处理,以及先用L.rhamnosus预处理,再用E.coli K88处理(D组).结果表明,B组细胞上清的T-AOC浓度显著降低(P＜0.01),SOD活力显著提高(P＜0.01),C组与此相反,并且细胞裂解液中的SOD活性显著提高(P＜0.05).B组和C组不同程度地促进APRIL的分泌(P＜0.01),而抑制IL-8的产生(P＜0.01).B组也抑制IL-10的产生,但C组却显著促进其分泌(P＜0.01).与B组相比,D组APRIL显著减少(P＜0.01),但IL-8和IL-10都显著增加(P＜0.05,P＜0.01).结果提示,L.rhamnosus可提高Caco-2细胞的抗氧化能力,减轻炎症反应,具有保护作用和免疫佐剂的效果.
|
| 关 键 词： | 鼠李糖乳杆菌 大肠杆菌K88 Caco-2细胞 抗氧化 细胞因子 |
Effects of Lactobacillus rhamnosus on Anti-oxidative Functions and Cytokines Secretion in Caco-2 Cells |
| |
| HUANG Yi
,
HUANG Qin
,
LI Wei-fen
,
YU Dong-you
,
ZHOU Xu-xia.Effects of Lactobacillus rhamnosus on Anti-oxidative Functions and Cytokines Secretion in Caco-2 Cells[J].Acta Veterinaria et Zootechnica Sinica,2012,43(2):250-254. |
| |
| Authors: | HUANG Yi HUANG Qin LI Wei-fen YU Dong-you ZHOU Xu-xia |
| |
| Institution: | 1 (1.Key Laboratory of Animal Molecular Nutrition of Ministry of Education,Colleg of Animal Science,Zhejiang University,Hangzhou 310058,China;2.College of Animal Science and Technology,Guangxi University,Nanning 530005,China) |
| |
| Abstract: | In this study,the effects of Lactobacillus rhamnosus on anti-oxidative and non-specific immunologic functions in Caco-2 cells were investigated.Caco-2 cells were treated by PBS(group A),E.coli K88(group B) and L.rhamnosus(group C),respectively.Caco-2 cells in group D were pre-incubated with L.rhamnosus and then infected with E.coli K88.The results showed that T-AOC concentration decreased(P<0.01),but SOD activity enhanced in cell culture supernatant(P<0.01)in group B.However,the adverse situation appeared in group C and,SOD activity in cell lysate increased(P<0.05).APRIL secretion were induced(P<0.01),however,IL-8 secretion were inhibited(P<0.01)both in group B and group C in different degree.IL-10 production was stopped in group B,and was promoted in group C(P<0.01).Compared with group B,APRIL production significantly decreased(P<0.01),whereas levels of IL-8(P<0.05)and IL-10(P<0.01)increased in group D.These results suggested that Caco-2 cells were sensitize to respond to L.rhamnosus by an improved response in anti-oxidative function.In addition,inflammatory response against pathogenic bacteria in enterocyte attenuated,moreover,immune response reinforced,which indicated the activities for immunoprotection and immunoadjuvant in L.rhamnosus. |
| |
| Keywords: | Lactobacillus rhamnosus Escherichia coli K88 Caco-2 cell antioxidant cytokine |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
