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| SLA-DQA基因在F18大肠杆菌抗性和敏感性断奶仔猪间的差异表达分析 | |
| 引用本文： | 訾臣,刘璐,苏先敏,杜子栋,黄雪根,杨建生,孙寿永,吴圣龙,包文斌.SLA-DQA基因在F18大肠杆菌抗性和敏感性断奶仔猪间的差异表达分析[J].畜牧兽医学报,2012,43(4):516-520. |
| 作者姓名： | 訾臣 刘璐 苏先敏 杜子栋 黄雪根 杨建生 孙寿永 吴圣龙 包文斌 |
| 作者单位： | 1. 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室,扬州,225009 2. 扬州大学兽医学院,扬州,225009 3. 江苏省现代种猪分子选育工程技术研究中心,常州213149;江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州225009 4. 江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州,225009 5. 扬州大学动物科学与技术学院江苏省动物遗传繁育与分子设计重点实验室,扬州225009;江苏省现代种猪分子选育工程技术研究中心,常州213149;江苏(扬州)规模猪场高效健康养殖公共技术服务中心,扬州225009 |
| 基金项目： | 转基因生物新品种培育科技重大专项，江苏省科技支撑计划，苏州市科技支撑计划，常州市科技攻关项目 |
| 摘 要： | 运用荧光定量PCR方法分析SLA-DQA基因在苏太猪F18大肠杆菌病抗性和敏感性资源群体中各个组织的分布和表达水平,以及在抗性组和敏感组中的表达差异,以探讨SLA-DQA基因在断奶仔猪抗大肠杆菌F18菌株感染中发挥的作用。试验结果显示,SLA-DQA基因在所检测的11个组织中均有表达,并呈现相似的表达规律,在肺、脾和淋巴结中的表达量较高,在空肠、十二指肠和胸腺中也有中度的表达。SLA-DQA基因在抗性组个体各个组织中的表达量普遍高于敏感性个体,而且在肺、脾、淋巴结、空肠和十二指肠5个组织中,SLA-DQA基因在抗性组个体中的表达量显著高于敏感性个体(P<0.05)。由此可见,当断奶仔猪受到大肠杆菌毒素侵扰后,SLA-DQA基因较高的表达量有利于SLA-II类抗原分子的合成,SLA-DQA基因虽然不是针对由大肠杆菌F18菌株造成的断奶仔猪腹泻和水肿病的直接免疫因子,但是在断奶仔猪受大肠杆菌F18菌株病原菌侵袭后引起的一系列生理变化和应答过程中发挥着重要的作用。 |
| 关 键 词： | 猪 F18大肠杆菌 SLA-DQA基因 荧光定量PCR |
The Differential Expression of SLA-DQA Gene between Weaning Piglets Resistant or Sensitive to Escherichia coli F18 |
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| ZI Chen , LIU Lu , SU Xian-min , DU Zi-dong , HUAN Xue-gen , YANG Jian-sheng , SUN Shou-yong , WU Sheng-long , BAO Wen-bin.The Differential Expression of SLA-DQA Gene between Weaning Piglets Resistant or Sensitive to Escherichia coli F18[J].Acta Veterinaria et Zootechnica Sinica,2012,43(4):516-520. | |
| Authors: | ZI Chen LIU Lu SU Xian-min DU Zi-dong HUAN Xue-gen YANG Jian-sheng SUN Shou-yong WU Sheng-long BAO Wen-bin |
| Institution: | 1,3,4 (1.Key Laboratory for Animal Genetics,Breeding,Reproduction and Molecular Design of Jiangsu Province,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009, China;3.Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149,China;4.Jiangsu(Yangzhou) Public Technical Service Centre for Efficient and Healthy Culture Technology in Large-scale Swine Farms, Yangzhou 225009,China) |
| Abstract: | The mRNA expression of SLA-DQA was assayed by real-time PCR in 11 different tissues in order to analyze the differential expression between resistant and sensitive post-weaning Sutai piglets invaded with ETEC F18 aiming to explore the role of SLA-DQA gene in the resistance against ETEC F18.The results showed that the SLA-DQA gene was broadly expressed in 11 tissues with a similar expression rule.The expression level was the highest in thymus,lung and lymph nodes.The expression level was moderate in the jejunum,duodenum and spleen.The mRNA expression of SLA-DQA gene in resistant piglets was higher than that in sensitive piglets generally.In lymph node,lung,spleen,jejunum and duodenum,the mRNA expression level of SLA-DQA gene in resistant piglets were significant higher than that in sensitive piglets(P<0.05).The analysis suggest that higher expression level of SLA-DQA could provide optimal conditions for the formation of the SLA Class II molecule when the weaning piglets were affected by E.coli F18-related toxins.SLA-DQA gene might not be the direct immune factor resisting the F18 of E.coli,but perhaps enhance humoral immunity and cell immunity to reduce the transmembrane signal transduction of ETEC F18 bacterial LPS and then led to the resistance to ETEC F18 in piglets. |
| Keywords: | piglets Escherichia coli F18 SLA-DQA gene real-time PCR |
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