| 多价噬菌体Bp7尾丝蛋白gp37的表达及其对噬菌体增殖的影响 |
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| 引用本文： | 吴德芹,颜晨,刘文华,温建新,邹玲,任慧英.多价噬菌体Bp7尾丝蛋白gp37的表达及其对噬菌体增殖的影响[J].畜牧兽医学报,2012,43(2):324-328. |
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| 作者姓名： | 吴德芹 颜晨 刘文华 温建新 邹玲 任慧英 |
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| 作者单位： | 青岛农业大学动物科技学院,青岛,266109 |
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| 摘 要： | 为研究大肠杆菌多价噬菌体Bp7的尾丝蛋白gp37在噬菌体宿主识别中的作用,通过PCR扩增噬菌体Bp7尾丝蛋白gp37的C末端基因,构建原核重组表达质粒pGEX-6p-1-gp37,并转化大肠杆菌BL-21,IPTG诱导表达。以鉴定正确的融合表达蛋白免疫家兔,制备多克隆抗体,并检测抗血清对噬菌体增殖作用的影响。结果显示:经SDS-PAGE及Western blot鉴定证实得到含有GST标签67ku的融合表达蛋白;抗Bp7尾丝蛋白gp37的抗血清可明显抑制噬菌体Bp3、Bp7的增殖(P<0.05),对噬菌体Bp2、Bp5、Bp6的增殖具有抑制作用,但差异不显著(P<0.05),该抗血清对噬菌体Bp4的增殖反而具有促进作用。试验结果证实gp37参与噬菌体Bp7的宿主识别。
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| 关 键 词： | 多价噬菌体 尾丝蛋白 原核表达 多克隆抗体 宿主识别 |
Expression of Tail Fiber Protein gp37 of Polyvalent Phage Bp7 and the Funtion in the Phage Reproduction |
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| WU De-qin
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YAN Chen
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LIU Wen-hua
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WEN Jian-xin
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ZOU Ling
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REN Hui-ying.Expression of Tail Fiber Protein gp37 of Polyvalent Phage Bp7 and the Funtion in the Phage Reproduction[J].Acta Veterinaria et Zootechnica Sinica,2012,43(2):324-328. |
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| Authors: | WU De-qin YAN Chen LIU Wen-hua WEN Jian-xin ZOU Ling REN Hui-ying |
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| Institution: | (College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China) |
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| Abstract: | The aim was to study the function of gp37 in the host recognition of phage Bp7.The C-terminal gene of tail fiber protein gp37 of phage Bp7 was amplified by PCR,and then was cloned into pGEX-6p-1 plasmid.The recombinant plasmid pGEX-6p-1-gp37 was transformed into BL21,and then induced by IPTG.The polyclonal antibodies against GST·gp37 were prepared and used to study phage reproduction.The results of SDS-PAGE and Western blot showed that a 67 kD GST-tagged fusion protein was obtained successfully.The polyclonal antibodies to gp37 of Bp7 could inhibit the reproductions of phages Bp3 and Bp7 obviously,while no significant effects on phages Bp2,Bp5 and Bp6 were detected.Antibodies to gp37 of Bp7 could facilitate the reproduction of phage Bp4.The results verified that gp37 participated in the host recognition of Bp7. |
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| Keywords: | polyvalent phage tail fiber protein prokaryotic expression polyclonal antibody host recognition |
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