| 中国美利奴羊不同MBL型个体感染绵羊肺炎支原体的免疫应答分析 |
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| 引用本文： | 张慧,赵宗胜,赵凤,余鹏,班谦,王遵宝.中国美利奴羊不同MBL型个体感染绵羊肺炎支原体的免疫应答分析[J].畜牧兽医学报,2012,43(2):306-313. |
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| 作者姓名： | 张慧 赵宗胜 赵凤 余鹏 班谦 王遵宝 |
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| 作者单位： | 新疆石河子大学动物科技学院,石河子,832000 |
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| 摘 要： | 为探讨中国美利奴羊不同甘露(聚)糖结合凝集素(MBL)型个体感染绵羊肺炎支原体的免疫应答变化,对4种不同MBL型个体进行MBL水平测定,选择其中20只作为对照组,50只为试验组,在相同饲养条件下试验组人工感染绵羊肺炎支原体,分别在攻毒前1 d(-1 d)、攻毒后1 d(1 d)、1周(7 d)、2周(14 d)、3周(21 d)用ELISA方法定量分析血清中TNF-α、IFN-γ、补体C1、C3水平.结果显示,A型和B型其MBL浓度较低,C型MBL浓度较高,与对照组相比,在攻毒后1周,A型和B型IFN-γ表达显著降低(P＜o.05),攻毒后2周,补体C3表达显著降低,TNF-α升高显著(P＜0.05)；与A型和B型相比,在攻毒后1周,C型IFN-γ表达增加(P＜0.05),在攻毒后2周,补体C3表达增加、TNF-α显著降低(P＜o.05),补体C1各组均不显著.结论:低血清MBL浓度与绵羊支原体肺炎有一定的相关性,不同基因型之间其TNF-α、IFN-γ、补体C1、C3水平有差异,低浓度MBL绵羊更易发生比较严重的炎症反应.
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| 关 键 词： | 中国美利奴羊 绵羊支原体肺炎 MBL 细胞因子 |
Immunological Response Analysis of Chinese Merino Sheep with Different MBL Genotype Infected with Mycoplasma pneumonia |
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| ZHANG Hui
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ZHAO Zong-sheng
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ZHAO Feng
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YU Peng
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BAN Qian
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WANG Zun-bao.Immunological Response Analysis of Chinese Merino Sheep with Different MBL Genotype Infected with Mycoplasma pneumonia[J].Acta Veterinaria et Zootechnica Sinica,2012,43(2):306-313. |
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| Authors: | ZHANG Hui ZHAO Zong-sheng ZHAO Feng YU Peng BAN Qian WANG Zun-bao |
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| Institution: | (College of Animal Science and Technology,Shihezi University,Shihezi 832000,China) |
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| Abstract: | The aim of this study was to analyze the immunological response to Mycoplasma pneumonia in Chinese Merino Sheep with different MBL genotype.MBL levels were measured in the four different individuals,50 healthy Chinese Merino sheep with different genotypes were artifically infected with M.pneumonia under the same raising condition,while 20 sheep were the control group,the content of TNF-α,IFN-γ,C1,C3 in the serum were quantitatively assayed by ELISA on 1 day before infection(-1 d),and on 1(the day of infection),7,14 and 21 day.The results showed that: 1)A and B type had low concentration of MBL,while C type’s MBL level was higher;2) One week after infection,the percentage of IFN-γ in the MBL A and MBL B was significantly lower than that of control group(P<0.05),two weeks later,the level of C3 was lower and the TNF-α was significantly higher(P<0.05).Compared with the MBL A and MBL B at one week after infection,the level of IFN-γ was significantly higher in the group of high concentration of MBL,two weeks later,the level of C3 increased and the level of TNF-α was significantly lower(P<0.05),the percentage of C1 had no significant difference among the groups(P>0.05).Conclusion: there maybe some correlation between the lower concentration of MBL plasma and mycoplasma pneumonia,while there were some difference among the level of TNF-α,IFN-γ,C1,C3,the low MBL ones were likely to have more severe inflammatory response. |
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| Keywords: | Chinese Merino Sheep Mycoplasma pneumonia Mannan-binding lectin Cytokines |
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