| 桑树有丝分裂染色体观察新法探索实验——酸解去壁低渗法 |
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| 引用本文： | 余茂德,柯益富,敬成俊,游松柏.桑树有丝分裂染色体观察新法探索实验——酸解去壁低渗法[J].蚕学通讯,1996(1). |
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| 作者姓名： | 余茂德 柯益富 敬成俊 游松柏 |
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| 作者单位： | 西南农业大学蚕桑丝绸学院 |
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| 摘 要： | 取桑芽、幼叶经前处理后,用1N HCl酸解处理材料15分钟,再进行低渗滴片,制备桑有丝分裂染色本标本.经观察,用此法制备的染色体标本,同样可以达到酶解去壁低渗法的观察效果,而比酶解去壁低渗法极大的节约实验费用;与常规切片法、压片法相比,其染色体制备效果好,且操作程序更简捷.将该方法称作桑树酸解去壁低渗法.
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| 关 键 词： | 桑树 有丝分裂染色体 酸解去壁低渗 |
Experiment with Exploring New Method of Observation on Mitotic Chromosome in Mulberry-Wall Digested with Acid and Low Osmotic Method |
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| Yu Mao-de Ke Yi-fu Jing Cheng-jun You Song-bo.Experiment with Exploring New Method of Observation on Mitotic Chromosome in Mulberry-Wall Digested with Acid and Low Osmotic Method[J].Newsletter of Sericultural Science,1996(1). |
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| Authors: | Yu Mao-de Ke Yi-fu Jing Cheng-jun You Song-bo |
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| Abstract: | In this paper, we explored a new method of preparing a slide for survey of plant chromosem in mitosis. Mulberry buds or tender leaves pretreated were treated 15 mm. with 1N HCl, then the cell suspension was droped on the slide by means of low osmotic method. the result is the same as that of wall digested with enzyme and low osmotic method, and it can make more experimental costs saved than that of mentioned method. The slide is much better than that of cutting sheet and pressuring sheet. The process for slide is simpler and more directive than other methods. The technique is called wall digested with acid and low osmotic method |
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| Keywords: | : Mulberry Mitotic chromosome Wall digested with acid and low osmotic method |
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