| 87份中熟棉种质资源亲缘关系和遗传多样性研究 |
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| 引用本文： | 张彦波,尹 国,路正营,孙 璐,李世云,杨玉枫.87份中熟棉种质资源亲缘关系和遗传多样性研究[J].中国农学通报,2020,36(6):82-88. |
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| 作者姓名： | 张彦波 尹 国 路正营 孙 璐 李世云 杨玉枫 |
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| 作者单位： | 邯郸市农业科学院,河北邯郸 056001 |
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| 基金项目： | 中棉所“棉花生物学国家重点实验室开放课题基金”(CB2018A17);河北省科技厅“河北省重点研发计划项目农业关键共性技术攻关专项” (18226327D)。 |
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| 摘 要： | 为了阐明不同中熟棉种质资源间的亲缘关系,为优势杂交组合选配亲本材料,最终得到目标优良种质及审定品种,利用SRAP分子标记技术对87份中熟棉品种（系）进行遗传多样性分析。从80对引物组合中筛选出多态性引物,用于供试材料的PCR扩增。结果显示,筛选出的17对多态性引物共扩增出168个位点,其中90个位点呈现多态性,平均每对引物产生5.29个多态性位点,多态率达53.57%。利用NTSYS-pc2.11软件数据分析显示,87份材料之间的相似系数为0.61~1.00,平均值为0.82。当遗传相似系数为0.73时,可将87份棉花材料分为两大类,其中第Ⅰ类群包含65个材料,第Ⅱ类群包含22个材料。遗传多样性分析表明本文中选用的中熟棉种质资源遗传基础比较狭窄,今后结合分子标记结果选用亲缘关系较远的材料作为杂交亲本,创制新的中熟棉种质资源。
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| 关 键 词： | 中熟棉 种质资源 SRAP 亲缘关系 遗传多样性 |
| 收稿时间： | 2019/9/16 0:00:00 |
| 修稿时间： | 2019/11/28 0:00:00 |
The Genetic Relationship and Diversity of 87 Mid-matured Cotton Germplasm Resources |
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| Zhang Yanbo,Yin Guo,Lu Zhengying,Sun Lu,Li Shiyun,Yang Yufeng.The Genetic Relationship and Diversity of 87 Mid-matured Cotton Germplasm Resources[J].Chinese Agricultural Science Bulletin,2020,36(6):82-88. |
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| Authors: | Zhang Yanbo Yin Guo Lu Zhengying Sun Lu Li Shiyun Yang Yufeng |
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| Institution: | Handan Academy of Agricultural Sciences, Handan Hebei 056001 |
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| Abstract: | To reveal the genetic relationship among different mid-matured cotton germplasms, select parent materials for superior hybrid combinations, and finally obtain the target superior germplasms and approved varieties, the genetic diversity of 87 cotton varieties or lines was studied using SRAP markers. The polymorphic SRAP primers were selected from 80 pairs of primer combinations for PCR amplification among the test materials. The results showed that a total of 168 loci were detected using 17 pairs of polymorphic SRAP primers, 90 of which were polymorphic, with an average of 5.29 polymorphic loci per primer, and the polymorphic rate was 53.57%. Data analysis by NTSYS-pc2.11 software showed that the genetic similarity coefficient of 87 cotton materials ranged from 0.61 to 1.00, with an average of 0.82. When the genetic similarity coefficient was given as 0.73, 87 cotton materials could be clustered into two groups, of which the first group included 65 materials and the second group included 22 materials. The genetic diversity analysis suggests that the genetic basis of mid-matured cotton is relatively narrow, and the materials with distant genetic relationship
should be selected as hybrid parents to create new mid-matured cotton germplasm resources in future. |
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| Keywords: | mid-matured cotton germplasm resources SRAP genetic relationship genetic diversity |
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