| HPLC法测定兽用除虫脲原料药含量及有关物质 |
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| 引用本文： | 左三龄,张博,邱银生,叶纯,吴仲元.HPLC法测定兽用除虫脲原料药含量及有关物质[J].中国兽药杂志,2020,54(2):46-52. |
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| 作者姓名： | 左三龄 张博 邱银生 叶纯 吴仲元 |
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| 作者单位： | 武汉轻工大学 动物科学与营养工程学院,,,,武汉轻工大学动物科学与饲料工程学院 |
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| 摘 要： | 建立了高效液相色谱法(HPLC)测定兽用除虫脲原料药含量及有关杂质的检测方法。采用C18色谱柱(250 mm×4.6 mm,5μm),以甲醇为流动相A,0.01 mol/L乙酸铵溶液为流动相B,进行梯度洗脱,流速1.0 mL/min,检测波长254 nm,进样量10μL,柱温25℃。除虫脲的线性范围为10~1000μg/mL(R 2=0.999),回收率97.01%~100.83%(RSD<1.0%,n=3),精密度(RSD=0.13%,n=9)、重复性(RSD=0.45%,n=6)良好,主成分与各杂质峰分离良好。以主成分自身对照法计算,各杂质峰面积的和不得大于对照溶液主峰面积的0.1倍(0.1%)。该方法简便灵敏,结果准确可靠,可作兽用除虫脲原料药含量及有关杂质测定。
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| 关 键 词： | 兽用 除虫脲 有关杂质 高效液相色谱法 |
| 收稿时间： | 2019/10/26 0:00:00 |
| 修稿时间： | 2020/1/13 0:00:00 |
Determination of the content and related substances in the veterinary diflubenzuron raw material by HPLC |
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| ZUO San-ling,ZHANG Bo,QIU Yin-sheng,YE Chun,WU Zhong-yuan.Determination of the content and related substances in the veterinary diflubenzuron raw material by HPLC[J].Chinese Journal of Veterinary Drug,2020,54(2):46-52. |
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| Authors: | ZUO San-ling ZHANG Bo QIU Yin-sheng YE Chun WU Zhong-yuan |
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| Institution: | (College of Animal Science and Nutritional Engineering,Wuhan Polytechnic University,Wuhan 430023,China) |
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| Abstract: | A method based on HPLC was developed for determination of the content and related substances of veterinary diflubenzuron.Using C18 column(250 mm x 4.6 mm,5μm)and methanol as mobile phase A,0.01 mol/L ammonium acetate solution as mobile phase B,with gradient elution.The flow rate was 1.0 mL/min.The UV detection at 254 nm.The injection volume was 10μL.The column temperature was 25℃.The results showed that linear range of the method was 10~1000μg/mL(R 2=0.999),the recovery rate of the preparation was between 97.01%and 100.83%(RSD<1.0%,n=3),with good precision(RSD=0.13%,n=9)and repeatability(RSD=0.45%,n=6).The main component and impurities could achieve a good separation.Calculated by the main component self-control method,the sum area of each impurity peak was not more than 0.1 times(0.1%)of the peak area of diflubenzuron in the control solution.This method was simple,sensitive,accurate and can be used for the determination of content and related impurities of veterinary diflubenzuron raw materials. |
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| Keywords: | veterinary diflubenzuron related substances HPLC |
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