| 拉萨市林周县白朗村农田土壤原核微生物多样性分析 |
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| 引用本文： | 靳贵梅,郭小芳.拉萨市林周县白朗村农田土壤原核微生物多样性分析[J].中国农学通报,2020,36(6):74-81. |
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| 作者姓名： | 靳贵梅 郭小芳 |
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| 作者单位： | 西藏大学理学院,拉萨 850000 |
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| 基金项目： | 国家重点研发计划项目子课题“不同恢复和利用方式下草地生态系统变化监测与评估”(2016YFC0502004-5);国家级大学生创新实验训练 计划项目“拉萨市林周县农田土壤微生物多样性分析”(201710694007);2016 年度西藏大学珠峰学者人才发展支持计划(藏大字[2016]141 号)。 |
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| 摘 要： | 开展拉萨市林周县农田土壤微生物多样性的研究,以期探究不同农田类型微生物群落结构差异。从林周县农田土壤分离细菌和放线菌,应用16S rDNA序列分析,并结合经典分类法对获得的菌株进行分类鉴定,确定农田土壤微生物群落结构。结果表明：细菌分属为6个属15个种,放线菌分属于13个种。出现频率和相对丰度显示,林周县白朗村农田土壤细菌优势种为Bacillus cereus和Bacillus simple,放线菌的优势种为Streptomyces pseudogriseolus、Streptomyces ciscaucasicus和Streptomyces flavogriseus。农作物类型对林周县白朗村农田土壤细菌和放线菌群落组成没有显著影响。
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| 关 键 词： | 青藏高原 农田土壤 土壤微生物细菌 放线菌 微生物群落 农作物类型 |
| 收稿时间： | 2018/11/9 0:00:00 |
| 修稿时间： | 2020/1/16 0:00:00 |
Diversity Analysis of Prokaryotic Microorganism of Farmland Soil: Bailang Village, Linzhou County, Lhasa |
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| Jin Guimei,De Ji,Yang Sang,Baima quwang,Gesang yuzhen,Guo Xiaofang.Diversity Analysis of Prokaryotic Microorganism of Farmland Soil: Bailang Village, Linzhou County, Lhasa[J].Chinese Agricultural Science Bulletin,2020,36(6):74-81. |
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| Authors: | Jin Guimei De Ji Yang Sang Baima quwang Gesang yuzhen Guo Xiaofang |
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| Institution: | School of Science, Tibet University, Lhasa 850000 |
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| Abstract: | The paper aims to explore the differences of microflora composition structure among different farmland types by studying the microorganism diversity of farmland soil in Linzhou, Lhasa. We isolated bacteria and actinomycetes from farmland soil and analyzed the 16S rDNA sequence, combined with the classic classification, we classified and identified the obtained strains, and finally determined the microflora composition structure of farmland soil. The results showed that soil bacteria were classified into 6 genera and 15 species, and actinomycetes were divided into 13 species; the occurrence frequency and relative abundance indicated that the dominant bacteria in soil were Bacillus cereus and Bacillus simple, however the dominant actinomyces were Streptomyces pseudogriseolus, Streptomyces ciscaucasicus and Streptomyces flavogriseus. Crop types have no significant influence on community composition of bacteria and actinomyces from farmland soil in Bailang village, Linzhou County. |
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| Keywords: | Tibetan plateau farmland soil bacteria actinomycetes microorganism community |
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