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马铃薯组培苗时期不同LED光源处理对温室移栽后植株生长和微型薯结薯的影响
引用本文:陈丽丽,王浩瑛,宫晓晨,高游慧,薛绪掌,胡跃高.马铃薯组培苗时期不同LED光源处理对温室移栽后植株生长和微型薯结薯的影响[J].中国农学通报,2020,36(6):11-18.
作者姓名:陈丽丽  王浩瑛  宫晓晨  高游慧  薛绪掌  胡跃高
作者单位:1. 宁波大学科学技术学院,浙江宁波 315300;2. 中国农业大学农学院,北京 100193;3. 北京农业智能装备技术研究中心,北京 100097
基金项目:国家高技术研究发展计划(863 计划)“基于网络管理的植物工厂智能控制关键技术研究”(2013AA103005)。
摘    要:不同LED光源下培养的马铃薯组培苗在形态特征上差异明显,为探索这种差异是否会对其移栽后产生进一步影响,试验研究了100%红光(R)、100%蓝光(B)、100%绿光(G)、75%红光+25%蓝光(RB)、45%红光+35%蓝光+20%绿光(RBG)和100%白光(CK)6种光谱下培养4周的马铃薯组培苗温室移栽后植株的生长和结薯情况。试验结果表明,B处理的马铃薯组培苗移栽后缓苗期叶绿素荧光参数Fv/FmqP、结薯前期SPAD值及结薯期各器官生物量均始终高于CK和其他处理,且该处理下最大薯的干鲜重和有效微型薯占比分别较CK提高45.66%、45.09%和10.93%。R处理的组培苗移栽后结薯期地上部生物量显著高于CK,单株结薯数目提高29.60%,但有效微型薯占比较CK降低63.97%。G处理的组培苗移栽后生长和结薯指标均表现最差。组合光谱RB和RBG处理的组培苗移栽后的生长指标和微型薯产量及产量构成指标均与CK无显著差异。因此,单色红、蓝、绿光谱处理对马铃薯组培苗的影响会持续至其移栽后植株幼苗生长,甚至结薯期,组合光谱RB和RBG则不会。在马铃薯组培苗生产上,单色蓝光和单色红光作为照明光源具有潜在的应用价值,组合光谱RB和RBG可作为白色光源的替代光源。

关 键 词:LED光源  马铃薯组培苗  微型薯生产  马铃薯植株生长  
收稿时间:2018/11/7 0:00:00
修稿时间:2020/2/10 0:00:00

Effects of LED Spectrum Treatments at in vitro Culture Stage on the Growth and Minituber Production of Potato Plantlets After Transplanting in Greenhouse
Chen Lili,Wang Haoying,Gong Xiaochen,Xue Xuzhang,Hu Yuegao.Effects of LED Spectrum Treatments at in vitro Culture Stage on the Growth and Minituber Production of Potato Plantlets After Transplanting in Greenhouse[J].Chinese Agricultural Science Bulletin,2020,36(6):11-18.
Authors:Chen Lili  Wang Haoying  Gong Xiaochen  Xue Xuzhang  Hu Yuegao
Institution:1. College of Science & Technology, Ningbo University, Ningbo Zhejiang 315300;2. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193;3. Research Center for Intelligent Equipment in Agriculture, Beijing 100097
Abstract:The morphology of potato plantlets grown in vitro under different LED light sources varies obviously. To explore whether the variations will have lasting effects on potato plantlets’ growth after transplanting in greenhouse, a single-factor randomized block design test was conducted with 4-week-old potato plantlets grown in vitro under 100% red light (R), 100% blue light (B), 100% green light (G), 75% red light+ 25% blue light (RB), 45% red light+ 35% blue light+ 20% green light (RBG) and 100% white (CK) light spectrum being transplanted in greenhouse to observe their growth and tuberization performance. The results indicated that chlorophyll fluorescence Fv/Fm and qP, SPAD value and biomass accumulation of potato plantlets from B treatment were always higher than that of CK and other treatments; the biggest minituber dry weight and fresh weight and the proportion of effective minituber in B treatment were increased by 45.66%, 45.09% and 10.93%, respectively, compared with CK. R treatment increased the number of minituber per plantlet by 29.60% but decreased the proportion of effective minituber by 63.97%. The parameters including Fv/Fm, qP, qN, SPAD, biomass accumulation in different organs and minituber yield and yield composition of plantlets from combined spectrum RB and RBG showed no significant differences from that of CK. However, in terms of both growth and tuberization, plantlets from G treatment performed the worst after transplanting. Thus, the effects of monochromic red, blue and green LED spectrum on the growth and tuberization of potato plantlets grown in vitro continued after the plantlets’ transplanting in greenhouse, whereas the combined spectrum RB and RBG did not. Thus, in the cultivation of potato plantlets in vitro, monochromatic blue and red LED spectrum have potential application value as light sources to provide illumination and combined spectrum RB and RBG can be utilized as the alternatives of white light.
Keywords:LED spectrum  tissue cultured potato plantlets  minituber production  potato plant growth
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