| 牛不同直径卵泡内卵母细胞体外受精后的卵裂率和染色体异常分析 |
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| 引用本文： | 朱淑文,华修国,艾晓杰,李培昌,吉泽绿.牛不同直径卵泡内卵母细胞体外受精后的卵裂率和染色体异常分析[J].中国兽医学报,2002,22(4):330-331. |
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| 作者姓名： | 朱淑文 华修国 艾晓杰 李培昌 吉泽绿 |
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| 作者单位： | 1. 上海交通大学,农学院,上海,201101
2. 日本国宇都宫大学,农学部,日本,宇都宫,321-8505 |
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| 基金项目： | 教育部博士点基金资助项目 (A2 95 9) |
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| 摘 要： | 将不同直径牛卵泡内卵母细胞体外成熟培养22h后，对其体外受精后的卵裂率，胚胎发育速度和胚胎染色体异常发生率进行了研究。结果表明，体外受精后48h，直径3－6mm卵泡内卵母细胞外受精后的卵裂率明显高于直径1－2mm和7－10mm卵泡内卵母细胞体外受精后的卵裂率（P＜0．05）。体外受精后48h，直径3－6mm包泡内卵母细胞体外受精后的胚胎的发育速度明显快于直径1－2mm和7－10mm卵泡组胚胎（P＜0．05）。通过对不同卵裂期胚胎的染色体标本分析表明，直径1－2mm卵泡内卵母细胞体外受精后的胚胎染色体异常发生率明显高于直径3－6mm卵泡组（P＜0．05），但与直径7－10mm卵泡组之间无显著性差异。
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| 关 键 词： | 体外受精 卵母细胞 体外成熟 卵裂率 染色体异常 卵泡直径 牛 |
| 文章编号： | 1005-4545(2002)04-0330-02 |
| 修稿时间： | 2002年1月22日 |
Analysis of Fertilizability and Chromosomal Aberrations in Bovine Follicular Ooctyes Matured in Vitro |
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| ZHU Shu wen ,HUA Xiu guo ,AI Xao jie ,LI Pei cang ,Yoshizawa Midori.Analysis of Fertilizability and Chromosomal Aberrations in Bovine Follicular Ooctyes Matured in Vitro[J].Chinese Journal of Veterinary Science,2002,22(4):330-331. |
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| Authors: | ZHU Shu wen HUA Xiu guo AI Xao jie LI Pei cang Yoshizawa Midori |
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| Institution: | ZHU Shu wen 1,HUA Xiu guo 1,AI Xao jie 1,LI Pei cang 2,Yoshizawa Midori 2 |
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| Abstract: | The purpose of the present experiment was to determine the fertilizability of in vitro matured bovine oocytes from follicles of different sizes.The results demonstrated that at 48 h after insermination,the cleavage rate of oocytes from 3 6 mm follicles was significantly higher than those of from 1 2 mm and 3 6 mm follicles( P <0 05).By analyzing the chromosomes of each stage of embryos,the frequency of chromosomal aberrations was significantly higher in oocytes from 1 2 mm follicles than that oocytes from 3 6 mm follicles( P <0 05),but not significantly different than that in oocyles from 7 10 mm follicles. |
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| Keywords: | oocytes in vitro maturation cleavage rate chromosomal aberration |
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