Development of EST-SSR markers in peanut (Arachis hypogaea L.) |
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| Authors: | Xinyan Wei Lifeng Liu Shunli Cui Huanying Chen Jingjing Zhang |
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| Institution: | (1) Crops Research Institute, Guangdong Academy of Agricultural Sciences, Wushan 510640 Guangzhou, PR, China;(2) USDA-ARS, Crop Protection and Management Research Unit, Tifton, Georgia, USA |
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| Abstract: | More molecular markers for potential use in peanut genetic research were developed. A total of 92403 EST sequences of peanut (Arachis) in the NCBI database were downloaded and analyzed. 2594 SSRs distributed in 2267 non-redundant EST sequences were detected, with tri-nucleotide motif (65.54%) as the most abundant motif type followed by di-nucleotide motif
(28.10%). Among the 92 repeat types, the top eight motif types were AG/TC (20.1%), AAG/TTC (11.8%), AAT/TTA (10.1%), AGG/TCC
(6.6%), AGA/TCT (6.3%), AT/TA (5.9%), ACT/TGA (3.8%), and ATG/TAC (3.7%) with higher frequency. A total of 237 primer pairs
were successfully designed based on the 2267 SSR-ESTs using DNA star software. |
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