| HP-20型大孔树脂富集纯化桑白皮总黄酮的工艺优选 |
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| 引用本文： | 杨晶,刘嘉琪,王宝昌,史丽颖,唐玲,王永奇.HP-20型大孔树脂富集纯化桑白皮总黄酮的工艺优选[J].安徽农业科学,2013(23):9610-9611,9638. |
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| 作者姓名： | 杨晶 刘嘉琪 王宝昌 史丽颖 唐玲 王永奇 |
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| 作者单位： | 大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622 |
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| 摘 要： | 目的]优选桑白皮总黄酮的富集工艺条件.方法]以总黄酮含量和洗脱率为指标,采用单因素试验考察HP-20型大孔树脂的静态饱和吸附量、动态饱和吸附量、乙醇浓度和乙醇用量等工艺条件.结果]桑白皮经醇提浓缩后,以HP-20型大孔树脂为吸附剂,以3 BV蒸馏水洗脱,浓度15％乙醇3 BV,浓度40％乙醇以2 BV/h流速进行洗脱,收集12 BV洗脱液,总黄酮纯度达到52.95％,总黄酮洗脱率为31.89％.结论]HP-20型大孔树脂能够有效的富集桑白皮总黄酮,工艺操作简单、效果好.
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| 关 键 词： | 桑白皮(CORTEX MORI) HP-20型大孔吸附树脂 总黄酮 工艺条件 |
Study on the Purification of Total Flavonoids from CORTEX MORI with HP-20 Macroporous Resin |
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| Institution: | YANG Jing et al (Institute of Materia Mediea of Dalian University, Dalian, Liaoning 116622) |
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| Abstract: | Objective ] To study on the purification of total flavonoids from CORTEX MORI. Method ] With the content of total flavonoids and the elution rate as index, the single-factor test was adopted to investigate technique conditions of static saturated adsorption capacity, dy- namic adsorption capacity, ethanol concentration and dosage of HP-20 maeroporous resin. Result ] HP-20 had the best separating efficiency when the volume of drug was 9 BV, washed with 3 BV of distilled water, and eluted with 3 BV of 15% ethanol and eluted with 12 BV of 40% ethanol. Under these process conditions, purity of total flavonoids was 52.95% , elution rate of total flavonoids was 31.89%. Conclusion] DiaionHP-20 maeroporous resin has good effect of purification of total flavonoids from CORTEX MORI. |
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| Keywords: | CORTEX MORI HP-20 macroporous resin Total flavonoids Technology condition |
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