| 正交试验法优选黄芪提取工艺 |
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| 引用本文： | 刘勇.正交试验法优选黄芪提取工艺[J].中兽医医药杂志,2003,22(3):10-11. |
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| 作者姓名： | 刘勇 |
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| 作者单位： | 甘肃定西扶正制药有限公司,甘肃,定西,743000 |
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| 摘 要： | 以黄芪甲甙为定量指标，研究黄芪的提取工艺。通过正交试验，用高效液相色谱法测黄芪甲甙含量进行优选。结果：乙醇浓度、用量及提取时间对黄芪甲甙的提取均有影响。最佳提取工艺为70％乙醇常压回流提取2次，第一次为药材量10倍，回流90min，第二次8倍量，回流60min；黄芪甲甙含量为0．095％。
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| 关 键 词： | 正交试验法 优选 黄芪 提取工艺 高效液相色谱法 含量测定 黄芪甲甙 |
| 文章编号： | 1000-6354(2003)03-0010-02 |
| 修稿时间： | 2003年1月13日 |
Optimization of extracting process of Astragalus P. E by orthogonal experimental design |
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| Liu Yong.Optimization of extracting process of Astragalus P. E by orthogonal experimental design[J].Journal of Traditional Chinese Veterinary Medicine,2003,22(3):10-11. |
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| Authors: | Liu Yong |
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| Abstract: | To study the extracting process of the active components of Astragalus P.E, the orthogonal assay was used for the dominant selection and astragalosides as quantitative standard for the content quantitation with HPLC determination. The result indicate that the concentration and quality of ethanol and time of extraction are the influent factors. The best process was set as follow: Astragalus P.E was refluxed 2 times with 70% alcohol in normal press, of which the quality of alcohol was 10 times of herbs at the first time, reflux for 90 min, and 8 times for 60 min at the second time. With the process established in the study, 0 095% astragalosides were extracted in the test. |
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| Keywords: | Astragalls P E HPLC Orthogonal assay |
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