| 三重复合诱变选育林可霉素高产菌株 |
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| 引用本文： | 毕国东,牛春.三重复合诱变选育林可霉素高产菌株[J].中国兽药杂志,2016,50(11):22-26. |
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| 作者姓名： | 毕国东 牛春 |
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| 作者单位： | 宁夏泰瑞制药股份有限公司,宁夏泰瑞制药股份有限公司 |
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| 摘 要： | 以提高林可霉素的生产水平为目的,将林可霉素产生菌LK15-012先用0.6%甲基磺酸乙酯(EMS)处理3 h,然后常压室温等离子体(ARTP-200W)照射90 s、最后紫外线(UV)照射50 s。结果表明:致死率为98.5%,正突变率为71.8%。最终获得2株高产突变株LK16-032和LK16-049,摇瓶效价比出发菌株LK15-012提高了42.50%和35.25%。将两株突变株分别通过100 L小试、10 t中试和60 t大生产进行发酵验证,发酵效价明显高于生产菌株。采用EMS-ARTP-UV三重复合诱变明显提高了林可霉素的生产水平。
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| 关 键 词： | 林可霉素 EMS-ARTP-UV三重复合诱变 高产突变株 |
| 收稿时间： | 8/4/2016 12:00:00 AM |
| 修稿时间： | 2016/8/26 0:00:00 |
Triple Breeding of Lincomycin Producing Strain |
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| Institution: | Terry Pharmaceutical Co., Ltd. Ningxia Institute of bacteria, |
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| Abstract: | In order to improve the level of production of lincomycin, clindamycin producing bacteria LK15-012 treated with 0.6% ethyl methyl sulfonic acid (EMS)3 h, the second with atmospheric pressure plasma (ARTP-200w) at room temperature irradiation of 90 s, finally by ultraviolet (UV) radiation 50 s. The results showed that the mortality rate was 98.5%, the positive mutation rate is 71.8%. Won 2 strains of high yield mutant strains LK16-032and LK16-049, shake flask titer than the starting strain LK15-012 increased by 42.50% and 35.25%.Will be high yield mutant strain respectively through the test in 100 L, 10 t pilot and 60 t fermentation carried out to verify the mass production, significantly higher than the production strains fermentation titer. The EMS-ARTP-UV triple compound mutagenesis lincomycin production levels are increased obviously. |
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