| 甜瓜雄全同株与纯雌株基因遗传分析及初步定位 |
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| 引用本文： | 刘威,盛云燕,马鸿艳,栾非时.甜瓜雄全同株与纯雌株基因遗传分析及初步定位[J].中国蔬菜,2010,1(4):24-30. |
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| 作者姓名： | 刘威 盛云燕 马鸿艳 栾非时 |
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| 作者单位： | 1.东北农业大学园艺学院，黑龙江哈尔滨 150030；;2.黑龙江八一农垦大学农学院，黑龙江大庆 163319 |
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| 基金项目： | 国家自然科学基金(30771466,30871723); 国家西甜瓜产业技术体系(nycytx-36-01-01-02); 黑龙江省杰出青年基金(jc200712) |
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| 摘 要： | 利用甜瓜纯雌系WI998与雄全同株品系TopMark配制杂交组合,通过对P1、P2、F1、F2、BC1P1、BC1P26个世代群体的遗传分析,对决定甜瓜性别表达基因进行研究;同时以F2分离群体为试材,采用SSR分子标记构建甜瓜遗传图谱,定位控制甜瓜性别表达基因。研究结果表明,控制甜瓜性别表达的基因有3个,分别为:雄全同株基因a、雌全同株基因g和纯雌系基因gy。初步构建了一个包括31个SSR标记和2个形态学标记的甜瓜遗传图谱,找到了两个与性别表达基因相关的SSR分子标记,其中MU55491与a基因的遗传距离为13.5cM,MU147232与gy基因的遗传距离为11.6cM。
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| 关 键 词： | 甜瓜 性别表达 SSR 基因定位 |
| 收稿时间： | 2009-06-17; |
Genetic Analysis and Initial Location of Melon(Cucumis melo L.)of Andromonecious and Gynoecious Genes |
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| LIU Wei,SHENG Yun-yan,MA Hong-yan,LUAN Fei-shi.Genetic Analysis and Initial Location of Melon(Cucumis melo L.)of Andromonecious and Gynoecious Genes[J].China Vegetables,2010,1(4):24-30. |
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| Authors: | LIU Wei SHENG Yun-yan MA Hong-yan LUAN Fei-shi |
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| Institution: | 1.Horticulture College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;2.Agronomy College,August First Farm Reclamation University,Daqing 163319,Heilongjiang,China |
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| Abstract: | Genetic analysis was carried out on P1,P2,F1,F2,BC1P1 and BC1P2 using WI998(gynoecy)and TopMark(andromonoecy)as hybridized combination.Genetic map was constructed by Simple Sequence Repeat Markers(SSR)and melon(Cucumis melo L.)genes determined sex expression were located using F2 generation as materials at the same time.The research indicated that a（andromonoecious）,g（gynomonoecious）and gy（gynoecious）were the three genes which determine sex expression of melon.A genetic map of melon included 31 SSR molecular markers and 2 morphological markers were constructed.Besides,two SSR molecular markers related with sex determination were found.The genetic distances were 13.5 cM between MU55491 marker and gene a and 11.6 cM between MU147232
marker and gene gy，respectively. |
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| Keywords: | Melon(Cucumis melo L ) Sex expression SSR Gene location |
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