|
|||||
|
|
| 日本脑炎病毒NS1基因在大肠杆菌中的高效表达 | |
| 引用本文： | 金吉东,仇华吉,田志军,周艳君,张守发,童光志.日本脑炎病毒NS1基因在大肠杆菌中的高效表达[J].中国兽医学报,2003,23(3):255-257. |
| 作者姓名： | 金吉东 仇华吉 田志军 周艳君 张守发 童光志 |
| 作者单位： | 1. 中国农业科学院,哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001;延边大学,农学院动物医学系,吉林,龙井,133400 2. 中国农业科学院,哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 3. 延边大学,农学院动物医学系,吉林,龙井,133400 |
| 摘 要： | 提取日本脑炎病毒弱毒株SA14-14-2的基因组RNA,用RT-PCR扩增其NS1基因cDNA,将其克隆到原核表达载体pET-30b(+)中,转化大肠杆菌BL-21(DE3),经IPTG诱导,NS1基因获得高效表达.SDS-PAGE分析显示,表达的融合蛋白表观相对分子质量约为46 000,重组NS1蛋白占菌体总蛋白的30.8%.Western blotting分析表明,重组蛋白具有良好的免疫原性. |
| 关 键 词： | 日本脑炎病毒 NS1基因 RT-PCR 表达 |
| 文章编号： | 1005-4545(2003)03-0255-03 |
| 修稿时间： | 2002年5月18日 |
High-level expression of Japanese encephalitis virus NS1 gene in E. Coli |
|
| JIN Ji dong ,QIU Hua ji ,TIAN Zhi jun ,ZHOU Yan jun ,ZHANG Shou fa ,TONG Guang zhi.High-level expression of Japanese encephalitis virus NS1 gene in E. Coli[J].Chinese Journal of Veterinary Science,2003,23(3):255-257. | |
| Authors: | JIN Ji dong QIU Hua ji TIAN Zhi jun ZHOU Yan jun ZHANG Shou fa TONG Guang zhi |
| Institution: | JIN Ji dong 1,2,QIU Hua ji 1,TIAN Zhi jun 1,ZHOU Yan jun 1,ZHANG Shou fa 2,TONG Guang zhi 1* |
| Abstract: | The cDNA of NS1 gene was amplified by RT PCR from Japanese encephalitis virus(JEV) genomic RNA,and then cloned into an expression vector pET 30b(+),resulting in a recombinant plasmid pET30b NS1.The recombinant plasmid was transformed into E.coli strain BL 21(DE3).A His fused recombinant protein with an apparent molecular weight of about 46 000 was obtained after induction by IPTG as indicated by SDS PAGE analysis,accounting for 30 8% of the total bacterial cellular lysates.The recombinant protein showed reactivity with both monoclonal antibody against His tag and specific antisera against JEV in western blotting. |
| Keywords: | Japanese encephalitis virus NS1 gene RT PCR expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |