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Cycas necrotic stunt virus isolated from gladiolus plants in Japan
Authors:Kaoru Hanada  Fumiyoshi Fukumoto  Manabu Kusunoki  Mitsuro Kameya-Iwaki  Yuko Tanaka  Toru Iwanami
Institution:(1) National Agricultural Research Center for Kyushu Okinawa Region, Kumamoto 861-1192, Japan;(2) National Agricultural Research Center, Tsukuba, Japan;(3) Forestry and Forest Products Research Institute, Tsukuba, Japan;(4) Yamaguchi University, Yamaguchi, Japan;(5) Present address: National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba 305-8666, Japan
Abstract:An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV. The nucleotide sequence data reported are available in the DDBJ/EMBL/Gen Bank databases under the accession number AB237656.
Keywords:Cycas necrotic stunt virus            Nepovirus  Gladiolus
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