Characterization of <Emphasis Type="Italic">Pectobacterium carotovorum</Emphasis> subsp. <Emphasis Type="Italic">carotovorum</Emphasis> causing soft-rot disease on <Emphasis Type="Italic">Pinellia ternata</Emphasis> in China |
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| Authors: | Xiu-Fang Hu Fei-Xiang Ying Yu-Bo He Yuan-Yuan Gao Hai-Min Chen Ji-Shuang Chen |
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| Institution: | (1) Institute of Bioengineering, Zhejiang Sci-Tech University, Road 2, Xiasha, Hangzhou, 310018, People’s Republic of China;(2) College of Life Science, Dalian Nationalities University, Dalian, 116600, People’s Republic of China |
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| Abstract: | Pinellia ternata is a traditional Chinese herb which has been used in China for over 1,000 years. A soft-rot disease characterized by water-soaked
lesions and soft-rot symptoms with a stinking odour was commonly observed in cultivated fields of this plant, and Pectobacterium-like bacteria were consistently isolated from the infected tissues. Two typical strains (SXR1 and ZJR1), isolated from Shanxi
and Zhejiang, respectively, were identified. Pathogenicity tests revealed that these strains were virulent to P. ternata and induced the same symptoms as observed in the field. Characterization involving fatty acid profile, metabolic and physiological
properties, 16S rDNA sequence and PCR-RFLP identified both isolates as P. carotovorum subsp. carotovorum (Pcc). The 16S rDNA of both isolates shared 97–99% sequence similarity with that of Pcc strains. The phylogenetic trees showed
that both isolates were clustered in the group of Pcc and P. carotovorum subsp. odorifera and both PCR-RFLP profiles were consistent with the pattern E produced by the minority of Pcc strains. Thus, isolates SXR1
and ZJR1 were characterized as Pcc in spite of some differences. This is the first report that Pcc has been proven as a causal
agent of soft-rot disease on P. ternata. |
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| Keywords: | Pinellia ternata Soft-rot disease Characterization Pathogen Pectobacterium carotovorum subsp carotovorum |
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