| 杏种子与种胚的超低温保存研究 |
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| 引用本文： | 李云,李嘉瑞.杏种子与种胚的超低温保存研究[J].种子,1995(6):14-16. |
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| 作者姓名： | 李云 李嘉瑞 |
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| 作者单位： | 西北农业大学园艺系，西北农业大学园艺系 陕西杨陵 712100，陕西杨陵 712100 |
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| 摘 要： | 含水量为６．９％带有内果皮的种子，以－５Ｃｍｉｎ＾－１速率降至－８０℃，放入ＬＮ２，在室温缓慢解冻，成活率可达９１．５％。去掉内果皮含水量为７．１％的种仁，以上述方式保存，成活率也可达到８８．２％。含水量是超低温保存杏种子最重要的因素。研究表明：冰冻保护剂ＤＮＳＯ对杏种子的超低温保存影响不大。含水量为４．８％￣８．１％去掉子叶的种胚超低温保存后ＴＴＣ染色成活率为８２．８％，并能在ＭＳ培养基上发育为
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| 关 键 词： | 杏树 种子 种胚 超低温保存 |
Cryopreservation of Embryocs and Seeds in Apricot (Primus ameniaca L. ) |
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| Li yun Li Jiarui.Cryopreservation of Embryocs and Seeds in Apricot (Primus ameniaca L. )[J].Seed,1995(6):14-16. |
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| Authors: | Li yun Li Jiarui |
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| Institution: | Northwestern Agricultural University. Shaanxi. Yang Ling. 712100 |
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| Abstract: | Apricot seeds with a moisture content 6.2%were cooled.to -80 C by a cooling rate of -50 min-1and put in liquid nitrogen (LN2)for storage. The living activity after thawing room tempreture (20 0-23C) could reach 91. 5%. The seeds without endocarp and with water content 7. 1% after treatment as mentioned abovet the living activity was 88. 2%. Seed water content was a key factor for stoage in LN2.The results showed that cryporeservation agent composed of 5-10% DMSO had a little effects for stored apricot seed in LN2. The embryoes stored in LN2 with removed cotyledons and water content 4. 8-8. 1 % , there viability by TTC inspection reached 82. 8%. embryoes after stored in LN2 could develop the plantlets in MS medium. |
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| Keywords: | Apricot Seed cryopreservation |
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