| 几种杂交蔬菜种子纯度鉴定指示同工酶的初步筛选 |
| |
| 引用本文： | 文方德,李卓杰.几种杂交蔬菜种子纯度鉴定指示同工酶的初步筛选[J].种子,1995(5):13-16. |
| |
| 作者姓名： | 文方德 李卓杰 |
| |
| 作者单位： | 中山大学生命科学学院!广州510275 |
| |
| 摘 要： | 该文以萌发３天的幼芽或胚轴为材料，用等电聚焦电泳技术分析研究番茄、辣椒、西瓜、青瓜和苦瓜杂交种子的酯酶（ＥＳＴ）、酸性磷酸酶（ＡＣＰ）、乙醇脱氢酶（ＡＤＨ）、苹果酸脱氢酶（ＭＤＨ）和谷草转氨酶（ＧＯＴ）同工酶，发现番茄种子的ＡＣＰ及ＡＤＨ同工酶、辣椒种子的ＡＣＰ及ＭＤＨ同工酶、西瓜种子的ＥＳＴ和ＡＣＰ同工酶、青瓜种子的ＡＣＰ同工酶以及苦瓜种子的ＡＣＰ和ＭＤＨ同工酶均有较强的品种特异性和稳定的酶谱，
|
| 关 键 词： | 同功酶 等电聚焦 纯度检测 蔬菜 种子 |
A Preliminary Selection of Isozmes as the Purity Testing Markers of Several Vegetable Hybrid Seeds |
| |
| Wen Fangde, Li Zhuojie, Fu Jiariu.A Preliminary Selection of Isozmes as the Purity Testing Markers of Several Vegetable Hybrid Seeds[J].Seed,1995(5):13-16. |
| |
| Authors: | Wen Fangde Li Zhuojie Fu Jiariu |
| |
| Abstract: | Five isogne systems, name1y esterase (EST), acid phophatase (ACP), a1cohol dehydrogenase (ADH), malatedehydrogenase (MDH) and glutamate oxaloacetase transaminase (GOT) from tomato, Pepper, watermelon, cucumter, palsam pear hybrids seeds, with sedling or plumular asis of germinating for 3 days seeds as the materials,were ana1y8ed by isoelectric focusing in order to select the marker isozymes fOr purity testing of these vegetable hybrids seeds. Thf isozyms electrophoretograms of ACP and ADH fbr tomato seeds, ACP and MDH for pepperseeds, EST and ACP for watermelon seeds, ACP for cucumber seeds, ACP and MDH for p1asam pear seeds werestable and had distinctive diffetentiation among each kind of varieties. These isozymes were Possibly suitable for indicating the purity identification of each vegetable hybrids yeds. |
| |
| Keywords: | isozymes isoelectric focusing purity testing vegetable hybrids seeds |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
