| 柞蚕抗菌肽的分离纯化及杀菌效应 |
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| 引用本文： | 邓小娟,黄自然.柞蚕抗菌肽的分离纯化及杀菌效应[J].华南农业大学学报,2002,23(3):13-16. |
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| 作者姓名： | 邓小娟 黄自然 |
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| 作者单位： | 华南农业大学艺术设计学院蚕丝科学系,广东,广州,510642 |
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| 摘 要： | 大肠杆菌（E.coli K12D31)诱导的柞蚕（Antheraea pernyi)蛹血淋巴，经加热处理后，进行CM－Sepharose阳离子交换层析，FPLC Pep RPC反相柱及HPLC Hi-Pore C18反相柱层析，纯化得到一类抗菌肽。SDS－聚丙烯酰胺凝胶电泳鉴定其相对分子质量约为2400，氨基酸组成分析表明其富含赖氨酸、丙氨酸和甘氨酸，摩尔分数分别为18．6％、15．5％和13．6％，不含甲硫氨酸、半胱氨酸、酪氨酸和组氨酸。抗菌谱表明该肽对多种革兰氏阴性和阳性细菌均有抑制作用。
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| 关 键 词： | 柞蚕 抗菌肽 分离纯化 杀菌效应 革兰氏阴性细菌 阳性细菌 |
| 文章编号： | 1001-411X(2002)03-0013-04 |
| 修稿时间： | 2001年9月18日 |
Isolation and Purification of Antibacterial Peptide from Chinese Oak Silkworm, Antheraea pernyi and Its Bactericidal Effects |
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| DENG Xiao juan,HUANG Zi ran.Isolation and Purification of Antibacterial Peptide from Chinese Oak Silkworm, Antheraea pernyi and Its Bactericidal Effects[J].Journal of South China Agricultural University,2002,23(3):13-16. |
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| Authors: | DENG Xiao juan HUANG Zi ran |
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| Abstract: | Injection of live nonpathogenic bacteria, Esherichia coli K 12 D 31 into pupae of the Chinese oak silkworm, Antheraea pernyi, induced antibacterial peptides in the hemolymph. The major antibacterial peptide was purified with CM-Sepharose cation-exchange chromatography, reverse phase chromatography on FPLC system and C 18 column reverse phase chromatography on HPLC system. The purified peptide had a molecular mass of about 2 400 as determined by SDS-PAGE and its amino acid composition was shown to be rich in lysine , alanine and glycine, while lacking in methionine , cysteine , tyrosine and histidine. The molar content of lysine, alanine and glycine was 18 6%,15 5%,13 6% respectively. Inhibitory spectrum showed that it was potent against several Gram-negative and Gram-positive bacteria. |
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| Keywords: | Chinese oak silkworm( Antheraea pernyi ) antibacterial peptide purification antibacterial effects |
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