| 两种体色瓯江彩鲤黑色素形成比较 |
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| 引用本文： | 余陆伟,曹思雨,王军,王成辉.两种体色瓯江彩鲤黑色素形成比较[J].上海海洋大学学报,2020,29(4):481-488. |
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| 作者姓名： | 余陆伟 曹思雨 王军 王成辉 |
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| 作者单位： | 上海海洋大学 农业农村部淡水水产种质资源重点实验室,上海 201306;上海海洋大学 水产科学国家级实验教学示范中心,上海 201306;上海海洋大学 上海水产养殖工程技术研究中心,上海 201306;上海海洋大学 农业农村部淡水水产种质资源重点实验室,上海 201306;上海海洋大学 水产科学国家级实验教学示范中心,上海 201306;上海海洋大学 上海水产养殖工程技术研究中心,上海 201306;上海海洋大学 农业农村部淡水水产种质资源重点实验室,上海 201306;上海海洋大学 水产科学国家级实验教学示范中心,上海 201306;上海海洋大学 上海水产养殖工程技术研究中心,上海 201306;上海海洋大学 农业农村部淡水水产种质资源重点实验室,上海 201306;上海海洋大学 水产科学国家级实验教学示范中心,上海 201306;上海海洋大学 上海水产养殖工程技术研究中心,上海 201306 |
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| 基金项目： | 国家自然科学(31372521)；上海市科委项目(15391900800)；广西科技重大专项(桂科AA17204095-7) |
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| 摘 要： |
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| 关 键 词： | 瓯江彩鲤 色素 mitfa 整胚原位杂交 |
| 收稿时间： | 2019/5/21 0:00:00 |
| 修稿时间： | 2020/2/19 0:00:00 |
Formation of melanin and mitfa in-situ in the Oujiang color common carp(Cyprinus carpio var.color) |
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| YU Luwei,CAO Siyu,WANG Jun,WANG Chenghui.Formation of melanin and mitfa in-situ in the Oujiang color common carp(Cyprinus carpio var.color)[J].Journal of Shanghai Ocean University,2020,29(4):481-488. |
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| Authors: | YU Luwei CAO Siyu WANG Jun WANG Chenghui |
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| Institution: | Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | In order to understand the formation of black pattern in Oujiang color common carp,learning the important function of pigment related gene. Futher to study the molecular mechanism of black patterns formation in Oujiang color common carp or other fishes. In this study, the melanin development was first observed in RB and WR. Meanwhile, the insitu probe of mitfa was prepared for analysing the exprssion and localization of mitfa gene in the important period of melanin development. According to the observation of the development of melanin in RB and WR in 9 development periods. In RB, the melanin appeared in the eyes at 36 hpf firstly, the melanin was first observed on the body at 3 dph, then melanin appeared locally at tail, abdomen and dorsal. In WR, the melanin only happened in the eyes, no melanin was observed in other position. Whole Mount in situ hybridizaton mitfa gene showed that the hybridization signal appeared on the head and abdomen where melanin appeared. With the melanin significantly increased at 10 dph, hybridization signal increases and more obvious. But even these is no melanin happened in the body of WR, the mitfa gene hybridization signal also can be observed. this study suggests that mitfa gene palys an important function of melanin in Oujiang color common carp,but it is not responsible for the difference of black pattern between the several Oujiang color common carp. |
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| Keywords: | Oujiang color common carp melanin mitfa in-situ |
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