| 乙酰胆碱酯酶试剂包配方的选择 |
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| 引用本文： | 王小瑜,王相友,孙霞,孙冬霞.乙酰胆碱酯酶试剂包配方的选择[J].保鲜与加工,2007,7(6):38-41. |
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| 作者姓名： | 王小瑜 王相友 孙霞 孙冬霞 |
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| 作者单位： | 山东理工大学轻工与农业工程学院,淄博,255049;山东理工大学轻工与农业工程学院,淄博,255049;山东理工大学轻工与农业工程学院,淄博,255049;山东理工大学轻工与农业工程学院,淄博,255049 |
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| 摘 要： | 以商品化乙酰胆碱酯酶试剂包为研究对象,比较了不同用量的乙酰胆碱酯酶、底物和显色剂下的抑制率,并在此基础上进行了正交试验,在保证一定的检测精度前提下最终确定了酶用量为原来的3/4,底物用量为原来的3/4,显色剂用量为原来的1/2,降低了检测成本。通过F检验证明,调整后配方与原配方之间无显著性差异。在此条件下,氧乐果、敌百虫、敌敌畏的检出下限大约分别为0.37mg/L、0.09mg/L、0.035mg/L,与原配方一致。
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| 关 键 词： | 乙酰胆碱酯酶 农药残留 检测限 |
| 文章编号： | 1009-6221（2007）06-0038-04 |
| 修稿时间： | 2007-07-09 |
Selection of Formulation of Acetylcholinesterase Reagent |
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| WANG Xiao-yu,WANG Xiang-you,SUN Xia,SUN Dong-xia.Selection of Formulation of Acetylcholinesterase Reagent[J].Storage & Process,2007,7(6):38-41. |
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| Authors: | WANG Xiao-yu WANG Xiang-you SUN Xia SUN Dong-xia |
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| Institution: | WANG Xiao-yu, WANG Xiang-you, SUN Xia, SUN Dong-xia (College of Light Industry ＆ Agricultural Engineering, Shandong University of Technology, Zibo 255049, China) |
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| Abstract: | The optimization of determination for organophosphate pesticide residues in vegetables was studied in this paper. In promise of higher sensitivity, a new low cost reagent components, whose dosage of acetylcholinesterase (ache), substrate and chromogenic reagent are 3/4, 3/4and 1/2 that of original components respectively, thus saved cost of reagent. The result was achieved through contrastive test. F-test proved that there is no significant difference between adjusted components and original components. The lower limit of determination of adjusted components to three pesticides is: omethoate 0.37 mg/L, trichlorphon 0.09 mg/L, dichlorvos 0.035 mg/L, which is consistent with those of national standard rapid determination method. |
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| Keywords: | ache pesticide residues detection limit |
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