| 香蕉ACC合成酶含3＇末端的cDNA克隆 |
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| 引用本文： | 金志强,徐碧玉,彭世清,孔德謇,郑学勤.香蕉ACC合成酶含3＇末端的cDNA克隆[J].热带作物学报,2001,22(1). |
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| 作者姓名： | 金志强 徐碧玉 彭世清 孔德謇 郑学勤 |
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| 作者单位： | 1. 中国热带农业科院
2. 华南热带农业大学农学院 |
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| 摘 要： | 采用3'RACE方法对香蕉(Musa AAA Cavendish Subgroup)ACC合成酶含3'末端的cDNA进行扩增,扩增产物被克隆到pCR*2.1载体上,转化大肠杆菌DH5α,筛选到重组质粒(pACS2),并对插入片段进行序列测定。结果表明,3'RACE产物长1 680 bp,其中一个开放读框内的核苷酸序列编码444个氨基酸,氨基酸序列包括了ACC合成酶中所应具有的7个高度保守区。同时该产物还有长269 bp的3'末端,并具有完整的Poly(A)尾(24mer)。
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| 关 键 词： | 香蕉ACC合成酶cDNA3'末端扩增 |
Cloning of a cDNA with Intact 3＇ end Encoding ACC Synthase in Banana （Musa AAA Cavendish Subgroup） |
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| Jin Zhiqiang,Peng Shiqing,Xu Biyu,Kong Dejian,Zheng Xueqin.Cloning of a cDNA with Intact 3＇ end Encoding ACC Synthase in Banana （Musa AAA Cavendish Subgroup）[J].Chinese Journal of Tropical Crops,2001,22(1). |
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| Authors: | Jin Zhiqiang Peng Shiqing Xu Biyu Kong Dejian Zheng Xueqin |
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| Abstract: | A fragment of cDNA with intact 3＇end which encodes ACC synthase in banana (Musa AAA Cavendish Subgroup) was obtained through rapid amplification of cDNA 3＇ end (3＇RACE). The product of PCR was cloned in the vector pCR? 2.1 and the recombinant plasmids was transformed to E.coli DH5a. The recombinant plasmid - pACS2 contains the insertion of the product of PCR. The sequence of the PCR product showed it was 1 680 bp long and encoded 444 amino acids whose sequence included all six highly conserved regions of ACC synthase. The PCR product also had 3＇ end of 269 bp long with intact polyA tail (24 mer). |
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