Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction |
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| Authors: | Duquesne F Pronost S Laugier C Petry S |
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| Institution: | AFSSA, Laboratoire d'Etudes et de Recherches en Pathologie Equine, IPC, Goustranville, 14430 Dozulé, France. |
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| Abstract: | A direct-PCR assay was developed for the rapid detection of Taylorella equigenitalis, a Gram-negative bacterium responsible for contagious equine metritis (CEM) in Equidae. The bacteria may be detected in equine genital swabs without need for a preliminary step of DNA extraction or bacterial isolation. Specificity was determined with 125 isolates of T. equigenitalis, 24 isolates of Taylorella asinigenitalis, five commensal bacteria of the genital tract and a facultative intracellular pathogen of foals found in large concentration in soil. Our PCR is specific and amplified a 413-bp 16S ribosomal DNA product only in all T. equigenitalis. |
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| Keywords: | Taylorella equigenitalis Contagious equine metritis Direct-PCR |
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