| 印楝茎尖组织培养的研究 |
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| 引用本文： | 陈芳,陈子牛,陆斌,马显达,陈娟.印楝茎尖组织培养的研究[J].西部林业科学,2003(1):8-11. |
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| 作者姓名： | 陈芳 陈子牛 陆斌 马显达 陈娟 |
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| 作者单位： | 1. 云南省林业科学院,云南,昆明,650204
2. 昆明师范专科学校,云南,昆明,650031 |
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| 摘 要： | 以印楝茎尖为外植体进行离体快速繁殖 ,外植体采用次氯酸钠预处理 ,用 0 1%的HgCl2 浸泡消毒 ,共设6 6个处理 ,3次重复 ,分 3次试验。结果表明 ,芽增殖培养基最佳组合为MS +6 -BA 7 0mg/L +IAA 0 0 1mg/L+ZT 0 1mg/L ,生根培养基为 1/ 2MS +NAA 0 5mg/L ,能有效促进芽生根 ,且移栽成活率可达 80 %以上
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| 关 键 词： | 印楝 茎尖 组织培养 |
| 文章编号： | 1007-3353(2003)01-0008-04 |
| 修稿时间： | 2002年5月23日 |
Study on Tissue Culture of Stem Apex of Azadirachta indica |
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| CHEN Fang ,CHEN Zi niu ,LU Bin ,MA Xian da ,CHEN Juan.Study on Tissue Culture of Stem Apex of Azadirachta indica[J].Journal of West China Forestry Science,2003(1):8-11. |
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| Authors: | CHEN Fang CHEN Zi niu LU Bin MA Xian da CHEN Juan |
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| Institution: | CHEN Fang 1,CHEN Zi niu 2,LU Bin 1,MA Xian da 1,CHEN Juan 1 |
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| Abstract: | The experiment of in vitro propagation of Azadirachta indica using the stem apex as explant was carried out. The explant was pre treated by sodium hypochlorite, soaking with HgCl 2 of 0 1 % in order to realize sterilization. Sixty treatments, and three replications were set up. The experimental results showed that the optimum preparations of bud enrichment and rooting were MS + 6-BA 7 0 mg/L + IAA 0 01 mg/L + ZT 0 1mg/L and 1/2 MS + NAA 0 5 mg/L respectively. The survival ratio of transplantation could achieve 80 %. |
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| Keywords: | Azadirachta indica stem apex tissue culture |
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