| 家蚕滞育卵中辅酶Ⅰ和辅酶Ⅱ的含量变化 |
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| 引用本文： | 赵林川,时连根.家蚕滞育卵中辅酶Ⅰ和辅酶Ⅱ的含量变化[J].蚕业科学,2008,34(3). |
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| 作者姓名： | 赵林川 时连根 |
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| 作者单位： | 浙江大学动物科学学院,杭州,310029;苏州大学生命科学学院,江苏苏州,215123;浙江大学动物科学学院,杭州,310029 |
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| 摘 要： | 利用苹果酸脱氢酶(MDH)和柠檬酸合酶(CS)串联反应以及葡萄糖-6-磷酸脱氢酶(G6PDH)催化葡萄糖-6-磷酸(G-6-P)脱氢反应,分别测定了家蚕滞育发动、即时浸酸和低温冷藏处理蚕卵辅酶Ⅰ(NAD+)和辅酶Ⅱ(NADP+)的含量。滞育发动时蚕卵NAD+含量显著下降,而即时浸酸和低温冷藏处理使蚕卵NAD+含量显著提高。蚕卵中的NADP+在滞育发动前大量积累,滞育发动时下降,即时浸酸处理后NADP+含量显著提高,在低温冷藏早期其含量进一步提高,低温冷藏处理后期又显著下降。分析上述结果认为,家蚕滞育卵中NAD+和NADP+含量的变化分别与呼吸耗氧量和糖元-山梨醇相互转化密切相关。
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| 关 键 词： | 家蚕 滞育卵 辅酶Ⅰ 辅酶Ⅱ |
Changes of NAD+ and NADP+ Content in Diapause Eggs of the Silkworm, Bombyx mori |
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| ZHAO Lin-Chuan,SHI Lian-Gen.Changes of NAD+ and NADP+ Content in Diapause Eggs of the Silkworm, Bombyx mori[J].Acta Sericologica Sinica,2008,34(3). |
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| Authors: | ZHAO Lin-Chuan SHI Lian-Gen |
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| Abstract: | Content of NAD+ and NADP+ in diapause eggs,common acid treatment eggs,and cold storage treatment eggs of the silkworm,Bombyx mori,were determined through the reactions catalysed by malic dehydrogenase,citrate synthase and glucosE-6-phosphate dehydrogenase,respectively.Content of NAD+ in eggs decreased remarkably during the onset of diapause,while increased significantly after common acid treatment or cold storage treatment.NADP+ exhibited a large number of accumulation near the onset of diapause,but henceforth decreased remarkably.An enhancement of NADP+content occurred after common acid treatment.For cold storage treatment,concentration of NADP+ further increased in the early stage while decreased significantly in the late stage.An enhancement of NADP+ content occurred after the treatment of hydrochloric acid.Above results showed that concentrations of NAD+ and NADP+ in the diapause eggs were closely related to changes of oxygen consumption and conversion of glycogen and sorbitol,respectively. |
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| Keywords: | Bombyx mori Diapause egg Nicotinamide adenine dinucleotide Nicotinamide adenine dinucleotide phosphate |
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